Detailed information of TA system TA222643

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3411283..3411926
• Replicon: chromosome
• Accession: NZ_CP086177
• Organism: Streptomyces fungicidicus strain F7

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: -
• Locus tag: LL040_RS15220
• Protein ID: WP_121546428.1
• Coordinates: 3411283..3411624 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: -
• Locus tag: LL040_RS15225
• Protein ID: WP_121546429.1
• Coordinates: 3411621..3411926 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LL040_RS15205	3407211..3408809	-	1599	WP_121546425.1	carboxyl transferase domain-containing protein	-
LL040_RS15210	3408806..3410482	-	1677	WP_121546426.1	acyclic terpene utilization AtuA family protein	-
LL040_RS15215	3410479..3411273	-	795	WP_121546427.1	TIGR03084 family metal-binding protein	-
LL040_RS15220	3411283..3411624	+	342	WP_121546428.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LL040_RS15225	3411621..3411926	+	306	WP_121546429.1	helix-turn-helix transcriptional regulator	Antitoxin
LL040_RS15230	3412001..3412981	-	981	WP_121546430.1	EamA family transporter	-
LL040_RS15235	3413240..3413692	+	453	WP_121546431.1	NUDIX domain-containing protein	-
LL040_RS15240	3413724..3416585	-	2862	WP_121546432.1	FAD-binding and (Fe-S)-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (21-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-88)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13063.15 Da
• Isoelectric Point: 11.4533

>T222643 WP_121546428.1 NZ_CP086177:3411283-3411624 [Streptomyces fungicidicus]
MAAPPKTIKHACWLLPRPQREVGPALRRPTVGAIERSRFRNMRELRPRNGGTVSIRMLFVFDPERRAIFLVAGNKATGRR
WASWYPRAIKEADDKYTAYLEALEQEKKEGQGR

Nucleotide

• Length: 342 bp

>T222643 NZ_CP117979:c2275481-2275329 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10854.41 Da
• Isoelectric Point: 6.4671

>AT222643 WP_121546429.1 NZ_CP086177:3411621-3411926 [Streptomyces fungicidicus]
MNLGSMDDLTADITPEKRERIDAIKAEMIDAERGHELAALRKAQGFTQVQVAKAMGVTQGRVSQIERGTARLDTSTMAAY
LHAIGGELTITATVGNLSVRL

Nucleotide

• Length: 306 bp

>AT222643 NZ_CP117979:c2276105-2275506 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAATATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA