Detailed information of TA system TA222616

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 149722..150224
• Replicon: plasmid pPA_DBM3797_2
• Accession: NZ_CP086135
• Organism: Pantoea agglomerans strain DBM 3797

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A8X8DPE2
• Locus tag: LKW31_RS22285
• Protein ID: WP_010256862.1
• Coordinates: 149970..150224 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A349IE86
• Locus tag: LKW31_RS22280
• Protein ID: WP_010256861.1
• Coordinates: 149722..149973 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LKW31_RS22265 (LKW31_22290)	145424..146875	+	1452	WP_191914321.1	flagellin	-
LKW31_RS22270 (LKW31_22295)	147217..148530	+	1314	WP_010256858.1	CitMHS family transporter	-
LKW31_RS22275 (LKW31_22300)	148698..149537	+	840	WP_159227283.1	MBL fold metallo-hydrolase	-
LKW31_RS22280 (LKW31_22305)	149722..149973	+	252	WP_010256861.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
LKW31_RS22285 (LKW31_22310)	149970..150224	+	255	WP_010256862.1	Txe/YoeB family addiction module toxin	Toxin
LKW31_RS22290 (LKW31_22315)	150280..152826	-	2547	WP_172608087.1	excinuclease ABC subunit UvrA	-
LKW31_RS22295 (LKW31_22320)	153490..153948	+	459	WP_172608114.1	hypothetical protein	-
LKW31_RS22300 (LKW31_22325)	153942..154235	+	294	WP_022624214.1	DUF1493 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..182426	182426

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9782.16 Da
• Isoelectric Point: 9.3799

>T222616 WP_010256862.1 NZ_CP086135:149970-150224 [Pantoea agglomerans]
VKLTWSAEAWEDYLYWQDADKKTVKKINELLKDASRTPFEGKGKPEPLKHNLAGFWSRRITAEHRLVYAVSGDALLIASC
RYHY

Nucleotide

• Length: 255 bp

>T222616 NZ_CP117972:c38812-38291 [Escherichia coli]
GTGAGCAATACAACGATAGAGATTTTCTCTGGAGAGAAAGATTATGATCTAAACGGTTTTGATTGCGGCGAAGAGTCACT
AAACGCCTTTTTAGCCAACCACTTAAAAAGGCAGCATGAGGGAAAAATTCTTCGTGCTTATGTGCTTTGCACTCAAGAAG
AAAGGCCAAAAGTGTTAGGATATTACACTTTGTCAGGTAGTTGTTTTGAAAAGGAGTCCTTACCTTCAAGAAGCAAACAA
AAGAAGGTTCCTTATCGGAATGTTCCAAGTGTTACTTTGGGTAGGCTGGCTTTGGACAAGTCCCTTCAAGGTCAAGGATT
TGGCTCAATGCTTGTAACACATGCAATGCGCGTTGTGTACAATGCATCTCTTGCTGTAGGCATTCATGGACTTTTCGTTG
AGGCGTTAAATGACAAAGCCAAGGCGTTTTATAAAAGTTTAGGCTTTATCCAGCTGGTTGGTAACAACGAGCGTTCTTTG
TTCTATCCTACAAAATCTATCGAAAAATTGTTCGAAGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9339.48 Da
• Isoelectric Point: 4.4155

>AT222616 WP_010256861.1 NZ_CP086135:149722-149973 [Pantoea agglomerans]
MRAISYSEARQNLSATMVQTVEDKAPVLITRQNGESCVLMSLEEYNSLEETAYLLRSPKNARRLMDSIESLQSGNGTDRD
IIE

Nucleotide

• Length: 252 bp

>AT222616 NZ_CP117972:c39089-38820 [Escherichia coli]
ATGTCAGCACTAAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATTACTCAGTTTATGGTTAGCAGTGCATCTGAACGTGCCGTGAAAGTTATAGAGCAACACCGTAGAC
TAGTTCTGAATGAAGAATCCTGGAATCTGGTTATGGACGCTATAAGCAATCCTCCGGCACCGAACGACAAGCTGAAACGA
GCTGCTGATCGTCTGAAAAGCATGGAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A349IE86