Detailed information of TA system TA222612

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 345479..346300
• Replicon: plasmid pPA_DBM3797_1
• Accession: NZ_CP086134
• Organism: Pantoea agglomerans strain DBM 3797

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: A0A379LR32
• Locus tag: LKW31_RS20610
• Protein ID: WP_045140153.1
• Coordinates: 345479..345949 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: A0A8X8DUC8
• Locus tag: LKW31_RS20615
• Protein ID: WP_033773852.1
• Coordinates: 345956..346300 (-)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LKW31_RS20600 (LKW31_20625)	340804..343206	+	2403	WP_228312684.1	maltodextrin phosphorylase	-
LKW31_RS20605 (LKW31_20630)	343215..345290	+	2076	WP_228312685.1	4-alpha-glucanotransferase	-
LKW31_RS20610 (LKW31_20635)	345479..345949	-	471	WP_045140153.1	type II toxin-antitoxin system YhaV family toxin	Toxin
LKW31_RS20615 (LKW31_20640)	345956..346300	-	345	WP_033773852.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
LKW31_RS20620 (LKW31_20645)	346399..346614	-	216	WP_157353015.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LKW31_RS20625 (LKW31_20650)	346803..347741	-	939	WP_228312686.1	maltose operon protein MalM	-
LKW31_RS20630 (LKW31_20655)	347844..349145	-	1302	WP_033760237.1	maltoporin	-
LKW31_RS20635 (LKW31_20660)	349189..350298	-	1110	WP_010247360.1	maltose/maltodextrin ABC transporter ATP-binding protein MalK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN	1..555522	555522

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-148)

Antitoxin

• PrlF antitoxin (15-113)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 18307.94 Da
• Isoelectric Point: 8.9277

>T222612 WP_045140153.1 NZ_CP086134:c345949-345479 [Pantoea agglomerans]
MEDSTVINGWHVYVHPCFESQLIALTNEVELLRAKYPESYQRKATTKLLAAVFKVINSEICADPHQAKFRQGDTLGEQNK
HWFRAKFLMQYRLFFRFSEQHKTIILAWMNDAETKRAYGSMRDAYKVFSGMLHNGYPPDDWALLLKQSQDWAKINS

Nucleotide

• Length: 471 bp

>T222612 NZ_CP117971:c4117144-4116746 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12285.96 Da
• Isoelectric Point: 4.3278

>AT222612 WP_033773852.1 NZ_CP086134:c346300-345956 [Pantoea agglomerans]
MGTLTHANISLRASSKLTARSQTTIPAAVRDAMNLQPGENIEYAVLPGGKVLITRQETVTDDDPVMASFLSFLAADIRNT
PANIKTLDRGLLTRITSLTEGMDVDLDAPLMDDE

Nucleotide

• Length: 345 bp

>AT222612 NZ_CP117971:c4117440-4117147 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A379LR32