Detailed information of TA system TA222602

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1805689..1806419
• Replicon: chromosome
• Accession: NZ_CP086133
• Organism: Pantoea agglomerans strain DBM 3797

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A356RQP5
• Locus tag: LKW31_RS08340
• Protein ID: WP_010670815.1
• Coordinates: 1806102..1806419 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LKW31_RS08335
• Protein ID: WP_010258577.1
• Coordinates: 1805689..1806021 (-)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LKW31_RS08315 (1801067)	1801067..1801708	+	642	WP_003849160.1	protein phosphatase CheZ	-
LKW31_RS08320 (1801897)	1801897..1803048	+	1152	WP_033772015.1	flagellar biosynthesis protein FlhB	-
LKW31_RS08325 (1803041)	1803041..1805134	+	2094	WP_124890046.1	flagellar biosynthesis protein FlhA	-
LKW31_RS08330 (1805134)	1805134..1805526	+	393	WP_010670816.1	flagellar protein FlhE	-
LKW31_RS08335 (1805689)	1805689..1806021	-	333	WP_010258577.1	HigA family addiction module antitoxin	Antitoxin
LKW31_RS08340 (1806102)	1806102..1806419	-	318	WP_010670815.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LKW31_RS08345 (1806494)	1806494..1807492	-	999	WP_228312638.1	aldo/keto reductase	-
LKW31_RS08350 (1807705)	1807705..1808640	-	936	WP_172608756.1	LysR family transcriptional regulator	-
LKW31_RS08355 (1808728)	1808728..1810458	-	1731	WP_010258588.1	arginine--tRNA ligase	-
LKW31_RS22420 (1810669)	1810669..1810791	-	123	WP_009089698.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (8-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12284.10 Da
• Isoelectric Point: 10.1171

>T222602 WP_010670815.1 NZ_CP086133:c1806419-1806102 [Pantoea agglomerans]
VATLRNIESFRDDWLKDYFLYGKFSKRIPSSLSSALARKLDIINAAISYKDLKSPPGNRYEELNPPLKGYASIRVNEQYR
LIFKWIEGKAVDLYLDAHSYKTHKR

Nucleotide

• Length: 318 bp

>T222602 NZ_CP117971:2127793-2128044 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12615.48 Da
• Isoelectric Point: 5.1473

>AT222602 WP_010258577.1 NZ_CP086133:c1806021-1805689 [Pantoea agglomerans]
MNQATRKPTTVGDVLLYEYLEPTGLKILDLAEMLKVHRNTVSALVNNNRKLTPDMAFRLAAAFETSVEFWLNLQNSVDIW
EVMHDARAQEEISRVTPLKDFLVQKENQLA

Nucleotide

• Length: 333 bp

>AT222602 NZ_CP117971:2128041-2128295 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A356RQP5