Detailed information of TA system TA222601

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1614644..1615304
• Replicon: chromosome
• Accession: NZ_CP086133
• Organism: Pantoea agglomerans strain DBM 3797

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A349IFT5
• Locus tag: LKW31_RS07455
• Protein ID: WP_010670918.1
• Coordinates: 1614644..1614997 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LKW31_RS07460
• Protein ID: WP_010247918.1
• Coordinates: 1615002..1615304 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LKW31_RS07435 (1609840)	1609840..1610121	+	282	WP_010247933.1	hypothetical protein	-
LKW31_RS07440 (1610220)	1610220..1610621	+	402	WP_010247930.1	cell envelope integrity TolA C-terminal domain-containing protein	-
LKW31_RS07445 (1610699)	1610699..1612861	-	2163	WP_228312600.1	ferric-rhodotorulic acid/ferric-coprogen receptor FhuE	-
LKW31_RS07450 (1613257)	1613257..1614345	+	1089	WP_029519619.1	YncE family protein	-
LKW31_RS07455 (1614644)	1614644..1614997	+	354	WP_010670918.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LKW31_RS07460 (1615002)	1615002..1615304	+	303	WP_010247918.1	XRE family transcriptional regulator	Antitoxin
LKW31_RS07470 (1615976)	1615976..1616899	+	924	WP_010247907.1	sugar ABC transporter substrate-binding protein	-
LKW31_RS07475 (1616932)	1616932..1618416	+	1485	WP_228312601.1	sugar ABC transporter ATP-binding protein	-
LKW31_RS07480 (1618440)	1618440..1619465	+	1026	WP_174819610.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-89)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13649.71 Da
• Isoelectric Point: 8.4949

>T222601 WP_010670918.1 NZ_CP086133:1614644-1614997 [Pantoea agglomerans]
VWMIKTTERFDRWFTLLNESDRACVLAALMVLREKGPGLSRPYADTLKGSVYINMKELRIQSRGDPIRAFFAFDPNRTAI
VLCAGNKVGNEKRFYQEMLPVADREFTHWLNSFKDEE

Nucleotide

• Length: 354 bp

>T222601 NZ_CP117971:1341105-1341440 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10941.72 Da
• Isoelectric Point: 5.7251

>AT222601 WP_010247918.1 NZ_CP086133:1615002-1615304 [Pantoea agglomerans]
MGRTLEQLIADEKPEVVADAQAMATDILLNIHLAELREKVQKTQVEMAKALGITQPTVAGMEKPGRDLKLSTLKRYIDAA
GGKLRLVVELPDGSHYGFEV

Nucleotide

• Length: 303 bp

>AT222601 NZ_CP117971:1340857-1341105 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A349IFT5