Detailed information of TA system TA222600

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 1588435..1589765
• Replicon: chromosome
• Accession: NZ_CP086133
• Organism: Pantoea agglomerans strain DBM 3797

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: LKW31_RS07335
• Protein ID: WP_228312590.1
• Coordinates: 1588435..1589457 (-)
• Length: 341 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: -
• Locus tag: LKW31_RS07340
• Protein ID: WP_124890102.1
• Coordinates: 1589454..1589765 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LKW31_RS07330 (1586090)	1586090..1588309	+	2220	WP_197979728.1	catalase/peroxidase HPI	-
LKW31_RS07335 (1588435)	1588435..1589457	-	1023	WP_228312590.1	HipA domain-containing protein	Toxin
LKW31_RS07340 (1589454)	1589454..1589765	-	312	WP_124890102.1	HipA N-terminal domain-containing protein	Antitoxin
LKW31_RS07345 (1589750)	1589750..1590085	-	336	WP_045140264.1	XRE family transcriptional regulator	-
LKW31_RS07355 (1590864)	1590864..1591985	+	1122	WP_010247976.1	Gfo/Idh/MocA family oxidoreductase	-
LKW31_RS07360 (1592076)	1592076..1592906	-	831	WP_033778851.1	glycosyltransferase family 8 protein	-
LKW31_RS07365 (1592919)	1592919..1594301	-	1383	WP_228312591.1	D-arabinono-1,4-lactone oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (58-302)

Antitoxin

• HipA, N-terminal subdomain 1 (5-98)

Sequences

Toxin

Protein

• Length: 341 a.a.
• Molecular weight: 38546.13 Da
• Isoelectric Point: 6.8875

>T222600 WP_228312590.1 NZ_CP086133:c1589457-1588435 [Pantoea agglomerans]
MNSERCLITLKRLKESELKTGYSTKGIRHLLGSLNVALSLPFTRSQFIQELPERQKGMSISGYQPKLSLRLAKGELVVVS
NDGTYILKPSPEEYPHLAENEHATMCVMQRLKFDVPPFGLMPFRHDENRPDELAFVIKRYDRTASDNARLHQEQLDGAMG
LGDKYGRIDNHKAISYEKAAKFLNTRVNSSLKSKRDLFLRIVYAYVLGNNDFHLRNIGIILPPQGSPWLAPVYDFISVVP
YPAAFGEYLALPLLEREENDAGNAPGMNSEYGEYLGADFILLAQGMDINPTLARKWLSEVVSHHQLIIDTYNESHMPESH
REAVLDYVARRITLLAVTEI

Nucleotide

• Length: 1023 bp

>T222600 NZ_CP117971:1204498-1204905 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11705.50 Da
• Isoelectric Point: 5.1019

>AT222600 WP_124890102.1 NZ_CP086133:c1589765-1589454 [Pantoea agglomerans]
MARKIEVWLYNTLAGTLSEGHNGYTFCYLPTYVGCAISLSMPVRPEPYLSEELHPFFKGLAPEGWLRKRYSEIQKIDDKD
LFGLLMENGNDLLGAVVFKEITS

Nucleotide

• Length: 312 bp

>AT222600 NZ_CP117971:1204251-1204517 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA