Detailed information of TA system TA222578

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5253542..5254137
• Replicon: chromosome
• Accession: NZ_CP086122
• Organism: Pseudomonas aeruginosa strain MIN-155

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: LLZ84_RS24290
• Protein ID: WP_003113526.1
• Coordinates: 5253859..5254137 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LLZ84_RS24285
• Protein ID: WP_003113527.1
• Coordinates: 5253542..5253847 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLZ84_RS24255 (LLZ84_24260)	5248655..5248945	-	291	WP_033992920.1	DUF5447 family protein	-
LLZ84_RS24260 (LLZ84_24265)	5249157..5249429	-	273	WP_003115921.1	hypothetical protein	-
LLZ84_RS24265 (LLZ84_24270)	5249555..5249824	+	270	WP_071536122.1	hypothetical protein	-
LLZ84_RS24270 (LLZ84_24275)	5249959..5251056	+	1098	WP_169917322.1	protein phosphatase 2C domain-containing protein	-
LLZ84_RS24275 (LLZ84_24280)	5251059..5252108	+	1050	WP_003113529.1	serine/threonine-protein kinase	-
LLZ84_RS24280 (LLZ84_24285)	5252177..5253178	+	1002	WP_228772168.1	serine/threonine-protein kinase	-
LLZ84_RS24285 (LLZ84_24290)	5253542..5253847	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
LLZ84_RS24290 (LLZ84_24295)	5253859..5254137	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LLZ84_RS24295 (LLZ84_24300)	5254190..5254318	-	129	Protein_4805	integrase	-
LLZ84_RS24300 (LLZ84_24305)	5254466..5256694	+	2229	WP_003113525.1	TonB-dependent receptor	-
LLZ84_RS24305 (LLZ84_24310)	5256764..5257411	-	648	WP_003095021.1	carbonate dehydratase	-
LLZ84_RS24310 (LLZ84_24315)	5257473..5258711	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T222578 WP_003113526.1 NZ_CP086122:c5254137-5253859 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T222578 NZ_CP117963:2097717-2097908 [Faecalibacterium prausnitzii]
ATGCCGATGACTCCCAAGCAAATGATTCGCTTGCTGGAGCAGAACGGCTTTGTTTTAGTCAGTGCAAATGGGTCACACTA
CAAGTACCATAACCCAACTACCAACCAAACCACTGTCGTTCCATTTCACGCAAAGGATCTGAAGCCCGGCACCGAGAAAA
ACATTCTCAAATTAGCCGGACTCAAAAAGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT222578 WP_003113527.1 NZ_CP086122:c5253847-5253542 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT222578 NZ_CP117963:2098049-2098426 [Faecalibacterium prausnitzii]
ATGAACGCTATTTTCTATCCCGCAATTTTTCACCCGGAAGAAACCGGGTACTCTGTTGAGATTCCCGATATTGAGGGCTG
CTTCACCCAAGGCGATACCATGGACGAAGCCGTCCGCATGGCACAGGATGCCATCGGCCTTATGCTGGAAGATTGCAAAG
TCTGCCCGGAGCCTTCTATCCCTTCTGCACTCCATGTTGACCCGGAAGATTTTGTGGTGATGGTGCCTTTCGATATGGAA
GAGTACGAAAAGCGATACAGACCCGTAAAGAAAACTCTTTCCATTCCCGGCTGGCTCAACGATGCCGCCGAATCGGCACA
CATCAACTTTTCCGGCGTCCTTCAGGATGCGCTGAAAGAGAAACTCCATTTGGCGTAA

Structures

Toxin

• AlphaFold DB: V6ALY3