Detailed information of TA system TA222568

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4786075..4786677
• Replicon: chromosome
• Accession: NZ_CP086118
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain S34

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C0Q3J8
• Locus tag: LLY43_RS23365
• Protein ID: WP_001159630.1
• Coordinates: 4786366..4786677 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LLY43_RS23360
• Protein ID: WP_000362050.1
• Coordinates: 4786075..4786365 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLY43_RS23345 (4783568)	4783568..4784470	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
LLY43_RS23350 (4784467)	4784467..4785102	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
LLY43_RS23355 (4785099)	4785099..4786028	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
LLY43_RS23360 (4786075)	4786075..4786365	-	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
LLY43_RS23365 (4786366)	4786366..4786677	-	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
LLY43_RS23370 (4786895)	4786895..4787824	+	930	WP_001127703.1	alpha/beta hydrolase	-
LLY43_RS23375 (4787910)	4787910..4788221	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
LLY43_RS23380 (4788218)	4788218..4788664	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
LLY43_RS23385 (4788679)	4788679..4789620	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
LLY43_RS23390 (4789665)	4789665..4790102	-	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
LLY43_RS23395 (4790099)	4790099..4790971	-	873	WP_000921427.1	virulence factor BrkB family protein	-
LLY43_RS23400 (4790965)	4790965..4791564	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12326.27 Da
• Isoelectric Point: 9.4460

>T222568 WP_001159630.1 NZ_CP086118:c4786677-4786366 [Salmonella enterica subsp. enterica serovar Typhimurium]
MQFIETELFTEDVKKLLDDDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T222568 NZ_CP117962:2923762-2924076 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT222568 WP_000362050.1 NZ_CP086118:c4786365-4786075 [Salmonella enterica subsp. enterica serovar Typhimurium]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT222568 NZ_CP117962:2924073-2924489 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A0R9NS64