Detailed information of TA system TA022256

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 448255..448858
• Replicon: plasmid pSMED02
• Accession: NC_009621
• Organism: Sinorhizobium medicae WSM419

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A6UKQ8
• Locus tag: SMED_RS27220
• Protein ID: WP_011970346.1
• Coordinates: 448583..448858 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: SMED_RS27215
• Protein ID: WP_011970345.1
• Coordinates: 448255..448554 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMED_RS27195	443930..445120	+	1191	WP_011970343.1	alpha-hydroxy-acid oxidizing protein	-
SMED_RS27200	445160..445891	-	732	WP_164923150.1	histidinol-phosphatase	-
SMED_RS27205	446147..447424	-	1278	Protein_412	threonine synthase	-
SMED_RS27210	447426..447922	+	497	Protein_413	IS110 family transposase	-
SMED_RS27215	448255..448554	-	300	WP_011970345.1	HigA family addiction module antidote protein	Antitoxin
SMED_RS27220	448583..448858	-	276	WP_011970346.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SMED_RS27225	449119..449715	-	597	WP_164923137.1	hypothetical protein	-
SMED_RS27230	450099..450293	+	195	WP_164923138.1	cupin domain-containing protein	-
SMED_RS32420	450281..450436	-	156	WP_018210435.1	hypothetical protein	-
SMED_RS27235	450598..450960	+	363	Protein_419	transposase	-
SMED_RS27240	451073..451945	+	873	WP_011970347.1	formate dehydrogenase accessory sulfurtransferase FdhD	-
SMED_RS27245	451938..452561	+	624	WP_011970348.1	molybdenum cofactor guanylyltransferase MobA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..1245408	1245408
	-	flank	IS/Tn	-	-	450664..451005	341

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-91)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10190.51 Da
• Isoelectric Point: 7.1350

>T22256 WP_011970346.1 NC_009621:c448858-448583 [Sinorhizobium medicae WSM419]
VIRSFKDAEAVARCKAPKGFPSDIVRSAVRKLTILNSAAALDDLRSPPGNRLEALKGDREGQHSNRINDQWRICFVWTDA
GIEDVEIVDYH

Nucleotide

• Length: 276 bp

>T22256 NC_009621:c448858-448583 [Sinorhizobium medicae WSM419]
GTGATCAGGTCATTTAAGGACGCCGAAGCCGTTGCGCGCTGCAAGGCACCAAAAGGCTTTCCTTCAGATATCGTGAGGTC
GGCCGTTCGTAAATTGACGATCCTGAACAGTGCTGCCGCTCTCGACGACTTGCGTTCCCCTCCGGGAAATCGACTTGAAG
CGCTGAAGGGCGATAGAGAAGGCCAACACTCAAACCGGATCAATGATCAATGGCGCATATGCTTCGTATGGACAGACGCC
GGTATCGAAGACGTCGAAATAGTTGACTACCATTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11475.41 Da
• Isoelectric Point: 8.6243

>AT22256 WP_011970345.1 NC_009621:c448554-448255 [Sinorhizobium medicae WSM419]
MTHILPHVHPGEILREEYLRPLNMSAGALAKKLNVPRTRIERIAAEQTPITTDTALRLAKYLRTTPEFWMNMQISFNLKT
EEIALRRELELIPEIDRAA

Nucleotide

• Length: 300 bp

>AT22256 NC_009621:c448554-448255 [Sinorhizobium medicae WSM419]
ATGACCCATATCCTTCCCCACGTCCATCCGGGCGAAATCCTCCGCGAGGAGTACCTACGTCCCCTTAACATGAGCGCTGG
CGCACTGGCGAAAAAGCTCAATGTTCCTCGCACCCGCATCGAACGTATAGCGGCGGAGCAAACACCGATCACGACGGATA
CCGCGTTGCGCCTCGCCAAATATCTCCGCACAACGCCGGAGTTTTGGATGAACATGCAGATCAGCTTCAACCTCAAGACA
GAGGAAATAGCACTTCGTCGCGAACTGGAGTTGATCCCGGAAATCGATAGAGCGGCATAG

Structures

Toxin

• AlphaFold DB: A6UKQ8