Detailed information of TA system TA222523

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 974736..975325
• Replicon: chromosome
• Accession: NZ_CP086106
• Organism: Skermanella mucosa strain KEMB 2255-438

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: JL100_RS04470
• Protein ID: WP_202681668.1
• Coordinates: 974736..975017 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JL100_RS04475
• Protein ID: WP_202681667.1
• Coordinates: 975032..975325 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JL100_RS04445 (JL100_004465)	970516..971613	-	1098	WP_202681673.1	class I SAM-dependent methyltransferase	-
JL100_RS04450 (JL100_004470)	971606..972454	-	849	WP_202681672.1	prolipoprotein diacylglyceryl transferase	-
JL100_RS04455 (JL100_004475)	972674..973048	+	375	WP_202681671.1	accessory factor UbiK family protein	-
JL100_RS04460 (JL100_004480)	973311..973817	+	507	WP_202681670.1	YbjN domain-containing protein	-
JL100_RS04465 (JL100_004485)	973821..974636	+	816	WP_202681669.1	pyrroline-5-carboxylate reductase	-
JL100_RS04470 (JL100_004490)	974736..975017	+	282	WP_202681668.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JL100_RS04475 (JL100_004495)	975032..975325	+	294	WP_202681667.1	HigA family addiction module antitoxin	Antitoxin
JL100_RS04480 (JL100_004500)	975392..976255	-	864	WP_202681666.1	RNA polymerase factor sigma-32	-
JL100_RS04485 (JL100_004505)	976453..977088	+	636	WP_202681664.1	TetR/AcrR family transcriptional regulator	-
JL100_RS04490 (JL100_004510)	977205..977657	+	453	WP_202681663.1	phasin family protein	-
JL100_RS04495 (JL100_004515)	977812..978147	+	336	WP_202681818.1	tRNA-binding protein	-
JL100_RS04500 (JL100_004520)	978289..979431	+	1143	WP_202681662.1	cache and HAMP domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10797.16 Da
• Isoelectric Point: 9.0715

>T222523 WP_202681668.1 NZ_CP086106:974736-975017 [Skermanella mucosa]
MIKSFGNQAAEATWQRRFIKALPNDIVKAAHRKLTQIHNARSLNDLRAPPGNRLEALSADRLGQHSIRINDQWRVCFEWR
DGDAYDVEIVDYH

Nucleotide

• Length: 282 bp

>T222523 NZ_CP117959:3056422-3056796 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10506.93 Da
• Isoelectric Point: 4.4419

>AT222523 WP_202681667.1 NZ_CP086106:975032-975325 [Skermanella mucosa]
MADFPPTHPGDVLGEDFLKPLGMSGSVLARSIDVPETLVGDLLDGRRGVTPDMALRLARYFGTSAEFWLGMQTTYDLETA
RDAVGAEIEARVRPRAA

Nucleotide

• Length: 294 bp

>AT222523 NZ_CP117959:3056001-3056375 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGATGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCGGTTTATCCGACACCGGCACCCGCAACCACCTCATAA