Detailed information of TA system TA222471

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1550834..1551515
• Replicon: chromosome
• Accession: NZ_CP086064
• Organism: Pseudomonas aeruginosa strain CCBH28525

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KK481_RS07485
• Protein ID: WP_015503432.1
• Coordinates: 1550834..1551199 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KK481_RS07490
• Protein ID: WP_016561576.1
• Coordinates: 1551192..1551515 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KK481_RS07455 (KK481_007460)	1546839..1547273	-	435	WP_003158601.1	RidA family protein	-
KK481_RS07460 (KK481_007465)	1547494..1548447	+	954	WP_003085661.1	LysR substrate-binding domain-containing protein	-
KK481_RS07465 (KK481_007470)	1548420..1549304	-	885	WP_019725847.1	LysR substrate-binding domain-containing protein	-
KK481_RS07470 (KK481_007475)	1549405..1549830	+	426	WP_003163196.1	VOC family protein	-
KK481_RS07475 (KK481_007480)	1549849..1550121	-	273	WP_003085667.1	hypothetical protein	-
KK481_RS07480 (KK481_007485)	1550328..1550570	+	243	WP_043884955.1	hypothetical protein	-
KK481_RS07485 (KK481_007490)	1550834..1551199	+	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KK481_RS07490 (KK481_007495)	1551192..1551515	+	324	WP_016561576.1	XRE family transcriptional regulator	Antitoxin
KK481_RS07495 (KK481_007500)	1551631..1551750	-	120	Protein_1478	IS5/IS1182 family transposase	-
KK481_RS07500 (KK481_007505)	1551745..1552137	+	393	Protein_1479	hypothetical protein	-
KK481_RS07505 (KK481_007510)	1552137..1553140	+	1004	Protein_1480	tyrosine-type recombinase/integrase	-
KK481_RS07510 (KK481_007515)	1553357..1553608	+	252	WP_124136459.1	hypothetical protein	-
KK481_RS07515 (KK481_007520)	1553638..1554504	+	867	WP_023081971.1	hypothetical protein	-
KK481_RS07525 (KK481_007530)	1555016..1555894	-	879	WP_003109435.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T222471 WP_015503432.1 NZ_CP086064:1550834-1551199 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T222471 NZ_CP117874:735987-736361 [Pectobacterium carotovorum subsp. carotovorum]
ATGCAACTGCTGTCATTGGTTGTGCATGGCCTATTAGTATTGCTCATTCTGCTGGCACCGTGGCCGGACGGCTACGCGTG
GCTATGGCTATGCCTGGTCACGATGGTGATGTTCGGTTTTATCCGTAGCCAACGGAATATTAAATCCCGACAGGGGGAAA
TCGTTCTGCTCAGCGAAACGACCCTGAACTGGCGGCAGCAGGAATGGCAGATCGTTAAACGACCGTGGCTGCTGAAGAAT
GGCGTATTACTGTCATTACAAGCGGTTAACGGTAAAGACAAGCAACAGCTGTGGCTGGCATCGGACAGCATGGGCGATGA
TGAATGGCGTCACTTGCGTCAAATTCTTTTGCAGCAGAAACACTGGGCAAGATAG

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11903.72 Da
• Isoelectric Point: 5.8900

>AT222471 WP_016561576.1 NZ_CP086064:1551192-1551515 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQSRGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT222471 NZ_CP117874:735701-735967 [Pectobacterium carotovorum subsp. carotovorum]
ATGGAAATCGATAATAAATCACGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAATTGGATATCGCGATCATGCCGTT
TTTTGAGCATGACTATGACACACTTAATGACACTGATAAGCGCACGTTTGTACGTTTGTTACAAAGCGACGATCCCGATT
TATTCAACTGGCTGATGAACCACGGTGAACCGGAGGACGGAGAGCTGAAACGCATGATTTCCCTTATTCAGACGCGAAAT
AAAGATCGTGGCCCTGTGGCGATGTGA