Detailed information of TA system TA222469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 821613..822208
• Replicon: chromosome
• Accession: NZ_CP086064
• Organism: Pseudomonas aeruginosa strain CCBH28525

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: KK481_RS04015
• Protein ID: WP_003113526.1
• Coordinates: 821613..821891 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KK481_RS04020
• Protein ID: WP_003113527.1
• Coordinates: 821903..822208 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KK481_RS03995 (KK481_003995)	817039..818277	+	1239	WP_003113524.1	dipeptidase	-
KK481_RS04000 (KK481_004000)	818339..818986	+	648	WP_003095021.1	carbonate dehydratase	-
KK481_RS04005 (KK481_004005)	819056..821284	-	2229	WP_003099265.1	TonB-dependent receptor	-
KK481_RS04010 (KK481_004010)	821432..821560	+	129	Protein_795	integrase	-
KK481_RS04015 (KK481_004015)	821613..821891	+	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KK481_RS04020 (KK481_004020)	821903..822208	+	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
KK481_RS04030 (KK481_004030)	822613..823713	-	1101	WP_003099270.1	redox-regulated ATPase YchF	-
KK481_RS04035 (KK481_004035)	823754..824338	-	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
KK481_RS04040 (KK481_004040)	824380..824994	-	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
KK481_RS04045 (KK481_004045)	825111..826052	-	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
KK481_RS04055 (KK481_004055)	826219..827067	-	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T222469 WP_003113526.1 NZ_CP086064:821613-821891 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T222469 NZ_CP117874:477578-477883 [Pectobacterium carotovorum subsp. carotovorum]
ATGCCACAAGTGATCGTTTCAGAACGCGCGAGGCGTGATTTGCAGCGTCTACAGGATTTTCTAAAAAACAAAAATGCGCT
GGCCGCGAAAAAGGCCGCTGAAGTTTTGATTCGGGGCATTCGTCAGTTAGAAGCGCTGCCGAATATCGGGCGACCAGTGG
AACATCTTCCCCTTGAATATCAAGAGTTGGTGATCGAATTCGGCAGCAGCGGGTATGTGATGCTTTACCGTCACGATGAG
CTGACCGGCAGAGTGGTGATACTGACCGTTAAACATCAAAAAGAAGCTGGTTATCAAAACCCGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT222469 WP_003113527.1 NZ_CP086064:821903-822208 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT222469 NZ_CP117874:477300-477590 [Pectobacterium carotovorum subsp. carotovorum]
ATGGCTGACACCACACGTCAAACCACGACGCTGCGCATCGATCAGGACATTAAAGAGCGACTGAAAACGCTGGCCGATGA
CCGCAACAGCTCAGCCCATGCGCTGATGCTGGAAGCGATTGCCGAATACGTTGAACGGGAAGAAAAACGCAGCCAGTATC
GTAAAGAGGCGTTGGCCGCGTGGGAAGACTATCAGGACACTGGCAAACATATCACCGCAGATGAGACCGTTACCTGGTTA
GAATCCTGGGGTTCTGGACATGAACAGGATGCGCCTGCATGCCACAAGTGA

Structures

Toxin

• AlphaFold DB: V6ALY3