Detailed information of TA system TA222438

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5588843..5589438
• Replicon: chromosome
• Accession: NZ_CP086010
• Organism: Pseudomonas aeruginosa isolate KB-PA_F19

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: LLH04_RS26355
• Protein ID: WP_003113526.1
• Coordinates: 5589160..5589438 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LLH04_RS26350
• Protein ID: WP_003113527.1
• Coordinates: 5588843..5589148 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLH04_RS26320 (LLH04_26335)	5583872..5584144	-	273	WP_124117649.1	DNA-binding protein	-
LLH04_RS26325 (LLH04_26340)	5584254..5584520	+	267	WP_023088595.1	hypothetical protein	-
LLH04_RS26330 (LLH04_26345)	5584576..5584911	+	336	WP_124117651.1	hypothetical protein	-
LLH04_RS26335 (LLH04_26350)	5584943..5585911	-	969	WP_124117653.1	hypothetical protein	-
LLH04_RS26340 (LLH04_26355)	5585908..5587275	-	1368	WP_124117655.1	ATP-binding protein	-
LLH04_RS26345 (LLH04_26360)	5587408..5588514	+	1107	WP_124117657.1	DNA cytosine methyltransferase	-
LLH04_RS26350 (LLH04_26365)	5588843..5589148	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
LLH04_RS26355 (LLH04_26370)	5589160..5589438	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LLH04_RS26360 (LLH04_26375)	5589491..5589619	-	129	Protein_5223	integrase	-
LLH04_RS26365 (LLH04_26380)	5589767..5591995	+	2229	WP_003099265.1	TonB-dependent receptor	-
LLH04_RS26370 (LLH04_26385)	5592065..5592712	-	648	WP_003095021.1	carbonate dehydratase	-
LLH04_RS26375 (LLH04_26390)	5592774..5594012	-	1239	WP_010793756.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T222438 WP_003113526.1 NZ_CP086010:c5589438-5589160 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T222438 NZ_CP117852:4325384-4325602 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT222438 WP_003113527.1 NZ_CP086010:c5589148-5588843 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT222438 NZ_CP117852:4324982-4325356 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: V6ALY3