Detailed information of TA system TA222421

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3077975..3078577
• Replicon: chromosome
• Accession: NZ_CP085987
• Organism: Salmonella enterica strain SZL 38

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C0Q3J8
• Locus tag: LKX03_RS15645
• Protein ID: WP_001159630.1
• Coordinates: 3078266..3078577 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LKX03_RS15640
• Protein ID: WP_000362050.1
• Coordinates: 3077975..3078265 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LKX03_RS15625 (3075468)	3075468..3076370	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
LKX03_RS15630 (3076367)	3076367..3077002	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
LKX03_RS15635 (3076999)	3076999..3077928	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
LKX03_RS15640 (3077975)	3077975..3078265	-	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
LKX03_RS15645 (3078266)	3078266..3078577	-	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
LKX03_RS15650 (3078795)	3078795..3079724	+	930	WP_001127703.1	alpha/beta hydrolase	-
LKX03_RS15655 (3079810)	3079810..3080121	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
LKX03_RS15660 (3080118)	3080118..3080564	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
LKX03_RS15665 (3080579)	3080579..3081520	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
LKX03_RS15670 (3081565)	3081565..3082002	-	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
LKX03_RS15675 (3081999)	3081999..3082871	-	873	WP_000921427.1	virulence factor BrkB family protein	-
LKX03_RS15680 (3082865)	3082865..3083464	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12326.27 Da
• Isoelectric Point: 9.4460

>T222421 WP_001159630.1 NZ_CP085987:c3078577-3078266 [Salmonella enterica]
MQFIETELFTEDVKKLLDDDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T222421 NZ_CP117851:c3761604-3761320 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGACTTATAAGCTGGCATTTAACGAATCTGCACTAAAAGAGTGGAAAAAACTGGGTCATACTATACAGGAACAGTTTAA
AAAAAAGTTACGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAGTGACACGACACAGAAGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT222421 WP_000362050.1 NZ_CP085987:c3078265-3077975 [Salmonella enterica]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT222421 NZ_CP117851:c3761842-3761594 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Structures

Toxin

• AlphaFold DB: A0A0R9NS64