Detailed information of TA system TA222381

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 51837..52591
• Replicon: chromosome
• Accession: NZ_CP085983
• Organism: Salmonella enterica strain SZL 31

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5F003
• Locus tag: LJY15_RS00310
• Protein ID: WP_000558166.1
• Coordinates: 51837..52148 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LJY15_RS00315
• Protein ID: WP_001259011.1
• Coordinates: 52145..52591 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJY15_RS00280 (47495)	47495..48397	+	903	WP_227969907.1	formate dehydrogenase subunit beta	-
LJY15_RS00285 (48394)	48394..49029	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
LJY15_RS00290 (49026)	49026..49955	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
LJY15_RS00295 (50002)	50002..50292	-	291	WP_000362050.1	DNA-binding transcriptional regulator	-
LJY15_RS00300 (50293)	50293..50604	-	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	-
LJY15_RS00305 (50822)	50822..51751	+	930	WP_021294279.1	alpha/beta hydrolase	-
LJY15_RS00310 (51837)	51837..52148	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	Toxin
LJY15_RS00315 (52145)	52145..52591	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
LJY15_RS00320 (52606)	52606..53547	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
LJY15_RS00325 (53592)	53592..54029	-	438	WP_023993693.1	D-aminoacyl-tRNA deacylase	-
LJY15_RS00330 (54026)	54026..54898	-	873	WP_000921423.1	virulence factor BrkB family protein	-
LJY15_RS00335 (54892)	54892..55491	-	600	WP_000965698.1	glucose-1-phosphatase	-
LJY15_RS00340 (55682)	55682..56485	-	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
LJY15_RS00345 (56519)	56519..57415	-	897	WP_001520529.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12432.40 Da
• Isoelectric Point: 9.5334

>T222381 WP_000558166.1 NZ_CP085983:51837-52148 [Salmonella enterica]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTFTQPEEMKRYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T222381 NZ_CP117838:c1073757-1073539 [Enterobacter sp. S-33]
ATGTCTGATAAACCACTCACAAAGATCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACGCTTGAACGCGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCCGCTGACCATCGTCTTGCTGAAC
TAACAATGAATAAGCTGTATGACAAAATCCCTACTTCTGTGTGGAAATTTGTCCGTTAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16734.08 Da
• Isoelectric Point: 6.6451

>AT222381 WP_001259011.1 NZ_CP085983:52145-52591 [Salmonella enterica]
MRTHRQMDATSAKKIVDTFSDAVKTVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT222381 NZ_CP117838:c1074159-1073785 [Enterobacter sp. S-33]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGATCCAACATCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCAACTTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATCGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGTTACGGCATTAACGCTCAGGATTTGCAAAAATGGCGTAAATCGGGAAATCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTGAGTCTTTCCTGTTAA

Structures

Toxin

• AlphaFold DB: A0A0R9ME90