Detailed information of TA system TA222380

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 50002..50604
• Replicon: chromosome
• Accession: NZ_CP085983
• Organism: Salmonella enterica strain SZL 31

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C0Q3J8
• Locus tag: LJY15_RS00300
• Protein ID: WP_001159630.1
• Coordinates: 50293..50604 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LJY15_RS00295
• Protein ID: WP_000362050.1
• Coordinates: 50002..50292 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJY15_RS00280 (47495)	47495..48397	+	903	WP_227969907.1	formate dehydrogenase subunit beta	-
LJY15_RS00285 (48394)	48394..49029	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
LJY15_RS00290 (49026)	49026..49955	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
LJY15_RS00295 (50002)	50002..50292	-	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
LJY15_RS00300 (50293)	50293..50604	-	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
LJY15_RS00305 (50822)	50822..51751	+	930	WP_021294279.1	alpha/beta hydrolase	-
LJY15_RS00310 (51837)	51837..52148	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
LJY15_RS00315 (52145)	52145..52591	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
LJY15_RS00320 (52606)	52606..53547	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
LJY15_RS00325 (53592)	53592..54029	-	438	WP_023993693.1	D-aminoacyl-tRNA deacylase	-
LJY15_RS00330 (54026)	54026..54898	-	873	WP_000921423.1	virulence factor BrkB family protein	-
LJY15_RS00335 (54892)	54892..55491	-	600	WP_000965698.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12326.27 Da
• Isoelectric Point: 9.4460

>T222380 WP_001159630.1 NZ_CP085983:c50604-50293 [Salmonella enterica]
MQFIETELFTEDVKKLLDDDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T222380 NZ_CP085983:c50604-50293 [Salmonella enterica]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT222380 WP_000362050.1 NZ_CP085983:c50292-50002 [Salmonella enterica]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT222380 NZ_CP085983:c50292-50002 [Salmonella enterica]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• AlphaFold DB: A0A0R9NS64