Detailed information of TA system TA222370

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3466102..3466704
• Replicon: chromosome
• Accession: NZ_CP085981
• Organism: Salmonella enterica strain SZL 30

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C0Q3J8
• Locus tag: LJY16_RS17245
• Protein ID: WP_001159630.1
• Coordinates: 3466102..3466413 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LJY16_RS17250
• Protein ID: WP_000362050.1
• Coordinates: 3466414..3466704 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJY16_RS17210 (3461215)	3461215..3461814	+	600	WP_000965698.1	glucose-1-phosphatase	-
LJY16_RS17215 (3461808)	3461808..3462680	+	873	WP_000921423.1	virulence factor BrkB family protein	-
LJY16_RS17220 (3462677)	3462677..3463114	+	438	WP_023993693.1	D-aminoacyl-tRNA deacylase	-
LJY16_RS17225 (3463159)	3463159..3464100	+	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
LJY16_RS17230 (3464115)	3464115..3464561	-	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
LJY16_RS17235 (3464558)	3464558..3464869	-	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
LJY16_RS17240 (3464955)	3464955..3465884	-	930	WP_021294279.1	alpha/beta hydrolase	-
LJY16_RS17245 (3466102)	3466102..3466413	+	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
LJY16_RS17250 (3466414)	3466414..3466704	+	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
LJY16_RS17255 (3466751)	3466751..3467680	-	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
LJY16_RS17260 (3467677)	3467677..3468312	-	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
LJY16_RS17265 (3468309)	3468309..3469211	-	903	WP_000331361.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12326.27 Da
• Isoelectric Point: 9.4460

>T222370 WP_001159630.1 NZ_CP085981:3466102-3466413 [Salmonella enterica]
MQFIETELFTEDVKKLLDDDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T222370 NZ_CP117823:2020087-2020368 [Xylella fastidiosa]
ATGCGGAGGATTAGCCAGACTGGCCAATTCAAGCGCGACTACAAGCGCGAAGCTAAGGGCCAGCATCGCGCCACGCTTGA
TGAGGAACTGATACACGTCTTGGAAGCCTTGACCTGTGATCATCCGTTGGAGCCACGCCACCACGATCACGCACTAACTG
GCGACTGGAAAGATCACCGTGATTGCCACATCAAACCCGACTTGGTACTGATATACCGGAAGCCCGACAACGAGACATTG
CAGCTCGTGCGCATTGGCTCACACAGCGAACTAGGTTTGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT222370 WP_000362050.1 NZ_CP085981:3466414-3466704 [Salmonella enterica]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT222370 NZ_CP117823:2019837-2020100 [Xylella fastidiosa]
ATGTCTGCAAACGCAGTTGTCCGTGCCCGCGTTGACGCGCACATCAAAGAAGAAGCATCAACCGTGCTGGCGACGATGGG
CCTAAGCGTGTCTGATGCGTTCCGTATGATGCTGACCCGCATTGCCCGCGAGAAGGCGCTGCCGTTCGAGCCGCTGGTGC
CGAACGAAACTACCATCGCAGCCATGCGTGAAGCCCGTGCTGGTCACCTCAAGAGCTTTGATAGTATTGAGGCGCTCATG
GCGGACTTGCATGCGGAGGATTAG

Structures

Toxin

• AlphaFold DB: A0A0R9NS64