Detailed information of TA system TA222332

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 1718678..1719333
• Replicon: chromosome
• Accession: NZ_CP085970
• Organism: Bordetella pertussis strain FDAARGOS_1542

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: -
• Locus tag: LK412_RS08270
• Protein ID: WP_047122780.1
• Coordinates: 1718678..1718929 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: Q7VY40
• Locus tag: LK412_RS08275
• Protein ID: WP_003809516.1
• Coordinates: 1718932..1719333 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK412_RS08245 (LK412_08245)	1713839..1714969	-	1131	WP_010930401.1	helix-turn-helix transcriptional regulator	-
LK412_RS08250 (LK412_08250)	1715204..1715785	+	582	WP_019247931.1	MOSC domain-containing protein	-
LK412_RS08255 (LK412_08255)	1715922..1716986	+	1065	WP_003809510.1	fructose-bisphosphate aldolase class II	-
LK412_RS08260 (LK412_08260)	1717071..1717517	-	447	WP_003819827.1	GFA family protein	-
LK412_RS08265 (LK412_08265)	1717742..1718623	+	882	WP_003809513.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
LK412_RS08270 (LK412_08270)	1718678..1718929	+	252	WP_047122780.1	type II toxin-antitoxin system MqsR family toxin	Toxin
LK412_RS08275 (LK412_08275)	1718932..1719333	+	402	WP_003809516.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
LK412_RS08280 (LK412_08280)	1719353..1719589	+	237	WP_010930404.1	DUF5993 family protein	-
LK412_RS08285 (LK412_08285)	1719597..1720076	+	480	WP_010930405.1	disulfide bond formation protein B	-
LK412_RS08290 (LK412_08290)	1720226..1720741	+	516	WP_019247930.1	5-(carboxyamino)imidazole ribonucleotide mutase	-
LK412_RS08295 (LK412_08295)	1720744..1721934	+	1191	WP_010930406.1	5-(carboxyamino)imidazole ribonucleotide synthase	-
LK412_RS08300 (LK412_08300)	1721954..1722991	+	1038	WP_023853543.1	L-threonylcarbamoyladenylate synthase	-
LK412_RS08305 (LK412_08305)	1723128..1724150	+	1023	WP_003809532.1	amino acid ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (10-82)

Antitoxin

• Antitoxin MqsA/HigA-2 (1-131)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 8952.52 Da
• Isoelectric Point: 9.7242

>T222332 WP_047122780.1 NZ_CP085970:1718678-1718929 [Bordetella pertussis]
IGGAATKKPGQKRRAGAAALGLDFSAMVEIVLALTPRDFHKSMTTHADHRIWQDVYCPVTAMGAVYLKLTVLDGVPIVSF
KEL

Nucleotide

• Length: 252 bp

>T222332 NZ_CP117816:c19855-19658 [Legionella pneumophila]
ATGAAAAGCGCAGAAATAATAAAAATAATTGAACGCGATGGTTGGGTGCAATGTCATCAGAAAGGAAGTCATTGCCAATT
TAAACATCCCACCAAAAAGGGTAGGGTGACAGTACCTCATCCTAAAAAAGATTTACCTATAAAAACAGTGGCCAGTATCT
TTAAACAAGCTGGTATTAATAAGGAGGATATAAAATGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14528.70 Da
• Isoelectric Point: 8.5005

>AT222332 WP_003809516.1 NZ_CP085970:1718932-1719333 [Bordetella pertussis]
MKCPVCGAAALARDTRDVPYSYKGETITIAAVTGDWCPACGENVLDGAESARVSEAMLAFNRLVNARRVDPGFIVRVRRK
LGLDQREAAALFGGGTNAFSRYENGKTRPPLALVKLLRLLDRHPELLEEVRAD

Nucleotide

• Length: 402 bp

>AT222332 NZ_CP117816:c19661-19257 [Legionella pneumophila]
ATGAGATATCCTGTAGTAATACATAAAGACGAACATAGTGATTTTGGTGTAATTGTCCCTGATATTCCTGGATGCTATTC
TTCTGGTAGCACTTATGATGAAGCTTTAACTAACGTAATTGAGGCAATTGAATGCCATTTGGAAGGATTATTAATAGATA
ATGAGCCTCTTCCAGTTGGTACAACAATTGACCGATGGATTAATGATGAAGAGTTTCAGGGCGGGGTATGGGCATTTGTT
GACATTGATTTAAGCCAAATATCGGGTAAAGCAAAACGCATTAATATCACTATGCCAGAGCGCATTTTAAGCCTCATCGA
TCTGTATGCCAAAAACCATGCGATAAAAAATCGTTCCTCCTTTTTGGCCGATGCAGCTCTGAGTTATATGGAAAGTCATA
AGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3LKS6