Detailed information of TA system TA222311

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1675013..1675652
• Replicon: chromosome
• Accession: NZ_CP085952
• Organism: Haemophilus influenzae strain FDAARGOS_1560

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: LK401_RS08510
• Protein ID: WP_005650215.1
• Coordinates: 1675347..1675652 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LK401_RS08505
• Protein ID: WP_005650217.1
• Coordinates: 1675013..1675336 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK401_RS08485 (LK401_08485)	1670632..1670805	-	174	WP_011272700.1	DUF5363 domain-containing protein	-
LK401_RS08490 (LK401_08490)	1670802..1672772	-	1971	WP_011961904.1	GNAT family N-acetyltransferase	-
LK401_RS08495 (LK401_08495)	1672777..1673118	-	342	WP_005652996.1	SirB2 family protein	-
LK401_RS08500 (LK401_08500)	1673180..1674850	-	1671	WP_005650218.1	energy-dependent translational throttle protein EttA	-
LK401_RS08505 (LK401_08505)	1675013..1675336	-	324	WP_005650217.1	HigA family addiction module antitoxin	Antitoxin
LK401_RS08510 (LK401_08510)	1675347..1675652	-	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LK401_RS08515 (LK401_08515)	1675977..1676597	+	621	WP_005694310.1	zinc transporter binding subunit ZevA	-
LK401_RS08520 (LK401_08520)	1676600..1677568	+	969	WP_038441067.1	zinc transporter permease subunit ZevB	-
LK401_RS08530 (LK401_08530)	1678183..1680222	+	2040	WP_038441065.1	excinuclease ABC subunit UvrB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T222311 WP_005650215.1 NZ_CP085952:c1675652-1675347 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T222311 NZ_CP117781:c1781197-1780919 [Burkholderia sp. FERM BP-3421]
ATGATCCAATCGTTTCGTTGCAGGAATACCGCCGCCTTGTTTGCCGGCGCGCCGCCTCCCGCGTTTCGCGCGTTCGAACG
CGTCGCCTGCCGCAAGCTTGCGATGCTCGACGCGGCGCACGCCCTGCGCGACCTGGCCGCACCGCCTGGAAACATGCTCG
AAGCCCTGTCGGGCGATCGGCGGCACCAGTACTGCATCCGGATCAACCAACGCTGGCGTCTCTGCTTCGAATGGACGGAA
AAAGGTCCGGCGCAGGTCGAGATCGTCGACTATCACTGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT222311 WP_005650217.1 NZ_CP085952:c1675336-1675013 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT222311 NZ_CP117781:c1780897-1780592 [Burkholderia sp. FERM BP-3421]
ATGAAAACCATCAACGCGATGCCCGCGATCCACCCGGGCGAGGTGCTGCGCGAGGAATTCCTCGCACCGCTCGCGCTCAG
CGTGAATGCGCTGTCCCTCGCCCTCGTCGTACCCGCCACGCGCCTGCACGAAATCGTCAAGGAGCGGCGCGGCATCACCG
CCGACACCGCCTATCGGCTCGCGCGCTATTTCGGCACGACCGCCGAGTTCTGGCTGAATCTCCAGATCGACTACGATCTC
AAGATCCTGCCGACCCGCGACGAGATCAACCGCAAGGTCGAGCCGCGCGAACCGCACGAGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A806EC70