Detailed information of TA system TA222292

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ(toxin)
• Location: 11139..11727
• Replicon: chromosome
• Accession: NZ_CP085947
• Organism: Helicobacter pylori strain FDAARGOS_1563

Toxin (Protein)

• Gene name: TfiT
• Uniprot ID: M3P300
• Locus tag: LK447_RS00070
• Protein ID: WP_000921941.1
• Coordinates: 11139..11420 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: TfiA
• Uniprot ID: B5Z829
• Locus tag: LK447_RS00075
• Protein ID: WP_000065301.1
• Coordinates: 11413..11727 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK447_RS00035 (LK447_00035)	6352..6615	+	264	WP_000578855.1	hypothetical protein	-
LK447_RS00040 (LK447_00040)	6612..6910	+	299	Protein_8	hypothetical protein	-
LK447_RS00045 (LK447_00045)	6910..7851	+	942	WP_000625134.1	ATPase, T2SS/T4P/T4SS family	-
LK447_RS00050 (LK447_00050)	7848..8303	+	456	WP_000795414.1	hypothetical protein	-
LK447_RS00055 (LK447_00055)	8300..8590	+	291	WP_000159819.1	hypothetical protein	-
LK447_RS00060 (LK447_00060)	8591..9148	+	558	WP_001167793.1	hypothetical protein	-
LK447_RS00065 (LK447_00065)	9149..10876	+	1728	WP_001208197.1	type IV secretory system conjugative DNA transfer family protein	-
LK447_RS00070 (LK447_00070)	11139..11420	-	282	WP_000921941.1	YafQ family addiction module toxin	Toxin
LK447_RS00075 (LK447_00075)	11413..11727	-	315	WP_000065301.1	hypothetical protein	Antitoxin
LK447_RS00080 (LK447_00080)	12009..13919	+	1911	WP_001162744.1	relaxase/mobilization nuclease domain-containing protein	-
LK447_RS00085 (LK447_00085)	13944..15116	-	1173	WP_000753636.1	hypothetical protein	-
LK447_RS00090 (LK447_00090)	15148..15432	-	285	WP_000394653.1	hypothetical protein	-
LK447_RS00095 (LK447_00095)	15515..16171	-	657	WP_000587407.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10891.80 Da
• Isoelectric Point: 9.5726

>T222292 WP_000921941.1 NZ_CP085947:c11420-11139 [Helicobacter pylori]
MLKVRTKKDFLKDFNKHILSGRITESDVTSVVDCLKEQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEIKKQENELV
LLRLDTHSELFKK

Nucleotide

• Length: 282 bp

>T222292 NZ_CP117750:4169563-4169814 [Enterobacter hormaechei]
ATGCGTGTTATTAGTTACAGTGAAGCCCGACAAAACCTGTCGGCAACAATGGTCCAGACGACGGAAGATCGCGCGCCGGT
GCTTATTACGCGCCAGAACGGTGAATCTTGTGTTCTGATGTCGCTGGAAGAGTACAACTCGCTTGAAGAAACCGCATACC
TGATGCGCTCCCCGGCAAACGCAAAACGTCTGATGAACTCTATCGAGAGCTTGAAATCTGGTGGCGGGAAGGAAAGGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12237.68 Da
• Isoelectric Point: 5.6761

>AT222292 WP_000065301.1 NZ_CP085947:c11727-11413 [Helicobacter pylori]
MSNIITDYSQYNEKQLHNFLNSIEKQLLKAEGDKNKAIKKIQECELQERMIRQVLAQKHSQEKEPTPSLLNTINSKDDPE
YDVSFGDFNDFLQIAKQERERHNA

Nucleotide

• Length: 315 bp

>AT222292 NZ_CP117750:4169811-4170065 [Enterobacter hormaechei]
GTGAAGATCACATGGTCTGAAGAGGCATGGGAAGACTACCTCTATTGGCAGGAGACCGATAAGCGCATGGTGAAGAAAAT
CAATGAGCTTATCAAAATCTCCTGTCGCACTCCGTTTGAGGGGATCGGCAAGCCAGAGCCATTAAAACATAACCTGGCTG
GCTTCTGGTCTCGCCGAATCACCTCTGAGCATCGCATGGTCTATGCCATCACAGAAGAAGCGATCCTCATAGCTGCGTGC
CGATATCACTACTGA

Structures

Toxin

• AlphaFold DB: M3P300

Antitoxin

• AlphaFold DB: B5Z829