Detailed information of TA system TA222279

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 211250..211785
• Replicon: plasmid p1
• Accession: NZ_CP085907
• Organism: Enterococcus faecium strain SC1762-D

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: R2PQC0
• Locus tag: LLE91_RS14855
• Protein ID: WP_002314366.1
• Coordinates: 211250..211522 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: Q3Y220
• Locus tag: LLE91_RS14860
• Protein ID: WP_002297422.1
• Coordinates: 211522..211785 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLE91_RS14815 (LLE91_14815)	206355..206804	-	450	WP_010729386.1	TlpA disulfide reductase family protein	-
LLE91_RS14820 (LLE91_14820)	206960..207502	-	543	WP_002298088.1	hypothetical protein	-
LLE91_RS14825 (LLE91_14825)	207777..208420	+	644	Protein_233	transposase	-
LLE91_RS14830 (LLE91_14830)	208496..209176	+	681	WP_002331383.1	IS6-like element IS1216 family transposase	-
LLE91_RS14835 (LLE91_14835)	209235..209423	-	189	Protein_235	hypothetical protein	-
LLE91_RS14840 (LLE91_14840)	209424..210281	-	858	WP_002292680.1	ParA family protein	-
LLE91_RS14845 (LLE91_14845)	210567..210854	-	288	WP_002300557.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LLE91_RS14850 (LLE91_14850)	210844..211173	-	330	WP_002292678.1	AbrB family transcriptional regulator	-
LLE91_RS14855 (LLE91_14855)	211250..211522	-	273	WP_002314366.1	Txe/YoeB family addiction module toxin	Toxin
LLE91_RS14860 (LLE91_14860)	211522..211785	-	264	WP_002297422.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LLE91_RS14865 (LLE91_14865)	212019..212927	+	909	Protein_241	IS982-like element ISEfm1 family transposase	-
LLE91_RS14870 (LLE91_14870)	212930..214105	-	1176	WP_002336205.1	MFS transporter	-
LLE91_RS14875 (LLE91_14875)	214108..216357	-	2250	WP_106017163.1	alpha-galactosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-aph(2'')	-	1..224638	224638
	-	inside	IScluster/Tn	-	-	197008..222386	25378

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (9-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-69)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10780.24 Da
• Isoelectric Point: 9.9724

>T222279 WP_002314366.1 NZ_CP085907:c211522-211250 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Nucleotide

• Length: 273 bp

>T222279 NZ_CP117749:c5007081-5006734 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGCGGCAGTGATCTTGGTTCTGCGACAGAAGCAGAGTGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9959.22 Da
• Isoelectric Point: 4.5370

>AT222279 WP_002297422.1 NZ_CP085907:c211785-211522 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Nucleotide

• Length: 264 bp

>AT222279 NZ_CP117749:c5007342-5007091 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGACTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Structures

Toxin

• AlphaFold DB: A0A829F704

Antitoxin

• AlphaFold DB: A0A829F006