Detailed information of TA system TA222271

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 211248..211783
• Replicon: plasmid p1
• Accession: NZ_CP085895
• Organism: Enterococcus faecium strain SC1762

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: R2PQC0
• Locus tag: LK894_RS14830
• Protein ID: WP_002314366.1
• Coordinates: 211248..211520 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: Q3Y220
• Locus tag: LK894_RS14835
• Protein ID: WP_002297422.1
• Coordinates: 211520..211783 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK894_RS14790 (LK894_14810)	206354..206803	-	450	WP_010729386.1	TlpA disulfide reductase family protein	-
LK894_RS14795 (LK894_14815)	206959..207501	-	543	WP_002298088.1	hypothetical protein	-
LK894_RS14800 (LK894_14820)	207776..208419	+	644	Protein_233	IS3-like element ISEfa8 family transposase	-
LK894_RS14805 (LK894_14825)	208495..209175	+	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
LK894_RS14810 (LK894_14830)	209234..209422	-	189	Protein_235	hypothetical protein	-
LK894_RS14815 (LK894_14835)	209423..210280	-	858	WP_002292680.1	ParA family protein	-
LK894_RS14820 (LK894_14840)	210565..210852	-	288	WP_002300557.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LK894_RS14825 (LK894_14845)	210842..211171	-	330	WP_002292678.1	AbrB family transcriptional regulator	-
LK894_RS14830 (LK894_14850)	211248..211520	-	273	WP_002314366.1	Txe/YoeB family addiction module toxin	Toxin
LK894_RS14835 (LK894_14855)	211520..211783	-	264	WP_002297422.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LK894_RS14840 (LK894_14860)	212017..212925	+	909	WP_002295743.1	IS982-like element ISEfm1 family transposase	-
LK894_RS14845 (LK894_14865)	212928..214103	-	1176	WP_002336205.1	MFS transporter	-
LK894_RS14850 (LK894_14870)	214106..216355	-	2250	WP_106017163.1	alpha-galactosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-aph(2'')	-	1..224636	224636
	-	inside	IScluster/Tn	-	-	197007..222384	25377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (9-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-69)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10780.24 Da
• Isoelectric Point: 9.9724

>T222271 WP_002314366.1 NZ_CP085895:c211520-211248 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Nucleotide

• Length: 273 bp

>T222271 NZ_CP117746:24333-24617 [Klebsiella pneumoniae]
ATGACCTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTCCATGGACGCAAAGACCAGTACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTAACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTGACCAAACACAGAAATTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9959.22 Da
• Isoelectric Point: 4.5370

>AT222271 WP_002297422.1 NZ_CP085895:c211783-211520 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Nucleotide

• Length: 264 bp

>AT222271 NZ_CP117746:24095-24343 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACCTTCAATGCAGG
AGAAGGTGCCCCGGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCATATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: A0A829F704

Antitoxin

• AlphaFold DB: A0A829F006