Detailed information of TA system TA222258

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 87823..88092
• Replicon: plasmid pFK8695-KPC-33
• Accession: NZ_CP085890
• Organism: Klebsiella pneumoniae strain 8695

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LKW25_RS27700
• Protein ID: WP_001372321.1
• Coordinates: 87967..88092 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	87823..87888 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LKW25_RS27670	83533..84060	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
LKW25_RS27675	84118..84351	+	234	WP_000006003.1	DUF905 family protein	-
LKW25_RS27680	84412..86435	+	2024	Protein_117	ParB/RepB/Spo0J family partition protein	-
LKW25_RS27685	86504..86938	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
LKW25_RS27690	86935..87654	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	87666..87890	+	225	NuclAT_0	-	-
-	87666..87890	+	225	NuclAT_0	-	-
-	87666..87890	+	225	NuclAT_0	-	-
-	87666..87890	+	225	NuclAT_0	-	-
-	87823..87888	-	66	-	-	Antitoxin
LKW25_RS27695	87876..88025	+	150	Protein_120	plasmid maintenance protein Mok	-
LKW25_RS27700	87967..88092	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LKW25_RS27705	88411..88707	-	297	Protein_122	hypothetical protein	-
LKW25_RS27710	89007..89303	+	297	WP_001272251.1	hypothetical protein	-
LKW25_RS27715	89414..90235	+	822	WP_001234445.1	DUF932 domain-containing protein	-
LKW25_RS27720	90532..91179	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
LKW25_RS27725	91456..91839	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LKW25_RS27730	92030..92716	+	687	WP_015059009.1	PAS domain-containing protein	-
LKW25_RS27735	92810..93037	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-33 / blaSHV-12 / blaCTX-M-65	-	1..143980	143980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T222258 WP_001372321.1 NZ_CP085890:87967-88092 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T222258 NZ_CP117741:c278370-277927 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT222258 NZ_CP085890:c87888-87823 [Klebsiella pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin