Detailed information of TA system TA222255

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 57571..57824
• Replicon: plasmid pFK8695-KPC-33
• Accession: NZ_CP085890
• Organism: Klebsiella pneumoniae strain 8695

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LKW25_RS27485
• Protein ID: WP_001312851.1
• Coordinates: 57675..57824 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	57571..57630 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LKW25_RS27445 (52953)	52953..53297	-	345	Protein_72	IS6-like element IS26 family transposase	-
LKW25_RS27450 (53349)	53349..54053	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LKW25_RS27455 (54078)	54078..54278	+	201	WP_072354025.1	hypothetical protein	-
LKW25_RS27460 (54298)	54298..55044	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
LKW25_RS27465 (55099)	55099..55659	+	561	WP_000139328.1	fertility inhibition protein FinO	-
LKW25_RS27470 (55791)	55791..55991	+	201	WP_015059022.1	hypothetical protein	-
LKW25_RS27475 (56377)	56377..56976	+	600	WP_032083981.1	PIN domain-containing protein	-
LKW25_RS27480 (57038)	57038..57370	+	333	WP_152916585.1	hypothetical protein	-
- (57571)	57571..57630	-	60	NuclAT_1	-	Antitoxin
- (57571)	57571..57630	-	60	NuclAT_1	-	Antitoxin
- (57571)	57571..57630	-	60	NuclAT_1	-	Antitoxin
- (57571)	57571..57630	-	60	NuclAT_1	-	Antitoxin
LKW25_RS27485 (57675)	57675..57824	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
LKW25_RS27490 (58108)	58108..58356	+	249	WP_000083837.1	replication regulatory protein RepA	-
LKW25_RS27495 (58471)	58471..58649	+	179	Protein_82	protein CopA/IncA	-
LKW25_RS27505 (58668)	58668..59525	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
LKW25_RS27510 (60464)	60464..61117	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
LKW25_RS27515 (61210)	61210..61467	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
LKW25_RS27520 (61400)	61400..61801	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
LKW25_RS27525 (62050)	62050..62465	+	416	Protein_87	IS1-like element IS1B family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-33 / blaSHV-12 / blaCTX-M-65	-	1..143980	143980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T222255 WP_001312851.1 NZ_CP085890:57675-57824 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T222255 NZ_CP117740:c5322572-5322189 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT222255 NZ_CP085890:c57630-57571 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin