Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 5224681..5225261 Replicon chromosome
Accession NZ_CP085885
Organism Pseudomonas sp. HN2

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag LIS66_RS23145 Protein ID WP_227974544.1
Coordinates 5224681..5224959 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LIS66_RS23150 Protein ID WP_227973107.1
Coordinates 5224974..5225261 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIS66_RS23125 (LIS66_23125) 5220257..5220424 - 168 WP_016773090.1 DUF2474 domain-containing protein -
LIS66_RS23130 (LIS66_23130) 5220509..5221516 - 1008 WP_042610525.1 cytochrome d ubiquinol oxidase subunit II -
LIS66_RS23135 (LIS66_23135) 5221520..5222965 - 1446 WP_042610526.1 cytochrome ubiquinol oxidase subunit I -
LIS66_RS23140 (LIS66_23140) 5223346..5224581 + 1236 WP_227973106.1 MFS transporter -
LIS66_RS23145 (LIS66_23145) 5224681..5224959 + 279 WP_227974544.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LIS66_RS23150 (LIS66_23150) 5224974..5225261 + 288 WP_227973107.1 HigA family addiction module antitoxin Antitoxin
LIS66_RS23155 (LIS66_23155) 5225266..5225817 - 552 WP_227973108.1 DJ-1/PfpI family protein -
LIS66_RS23160 (LIS66_23160) 5225884..5226330 - 447 WP_064364089.1 DUF4879 domain-containing protein -
LIS66_RS23165 (LIS66_23165) 5226551..5227846 + 1296 WP_064364088.1 NCS2 family permease -
LIS66_RS23170 (LIS66_23170) 5227843..5228922 + 1080 WP_227973109.1 tRNA (uridine(54)-C5)-methyltransferase TrmA -
LIS66_RS23175 (LIS66_23175) 5228977..5229423 - 447 WP_227973110.1 DUF2442 domain-containing protein -
LIS66_RS23180 (LIS66_23180) 5229420..5229848 - 429 WP_083353612.1 DUF4160 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-91)

Antitoxin

Cro/C1-type helix-turn-helix domain

(20-60)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10776.36 Da        Isoelectric Point: 9.8953

>T222233 WP_227974544.1 NZ_CP085885:5224681-5224959 [Pseudomonas sp. HN2]
MIKSFQHKGLRGFYETGTTRGIRADHAKRLSRMLQFMDRATLPGDLDLPGWRLHPLKGELSEYWSLSVSGNWRVIFRFVG
SDIELVDYLDYR

Download         Length: 279 bp

>T222233 NZ_CP117730:42927-43268 [Klebsiella grimontii]
ATGTGGGATGTTGAAACAACGGAGGTTTTCGACAAGTGGTTTGAGGCGCAGATCGAAGCGCTTAAAGAGGATATGCTCGC
CGCTATGGTGATCTTATCCGAGTATGGCCCCCAACTCGGAAGGCCGTTTGCGGATACAGTAAACGATTCTGCTTTTTCGA
ATATGAAAGAGCTGCGCGTTCAGCATCGAGGTAGCCCGATCAGAGCTTTCTTTGTATTTGACCCTTCTCGTCGCGGCATC
GTGTTATGCGCCGGAGATAAAACAGGGTTAAATGAAAAGAGATTCTATAAAGATATGATCAAGCTTGCAGATGCTGAGTA
CCGTAAGCACCTTAATCAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10483.04 Da        Isoelectric Point: 6.6962

>AT222233 WP_227973107.1 NZ_CP085885:5224974-5225261 [Pseudomonas sp. HN2]
MPMHNPPHPGETLLLDVLPELGISVSELARHLGFARPHLSRVLHGHAPISPDLAVRLERAGIGKARVWLGGQTDYDLWQA
EHREQPMIEPLAAHG

Download         Length: 288 bp

>AT222233 NZ_CP117730:43278-43577 [Klebsiella grimontii]
ATGGCGACCCTTAAAGAATTAATGGCCAAACAGAGCCCGGAAAGCCAGGCGCGTATTGCTGAAAAAGTCGAAGAAATGCG
CCAGGCTATCGCATTAAGTATGTTAAGGGAAGAACTCAATATGTCTCAGGCAGAGCTGGCTAAGGCAATGGGCGTTAAAC
AACCCACCATTGCAAAAATGGAACAGGCTGATAACGACCCGCGTTTGTCAACGCTGAAGCGTTATGTTGTAGCGCTCGGG
GGGGAACTCAGTATTGATGTTAAGCTGCCAACCGGTAAGCGAATCGCATTCCATCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References