Detailed information of TA system TA222226

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 294184..294788
• Replicon: chromosome
• Accession: NZ_CP085885
• Organism: Pseudomonas sp. HN2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LIS66_RS01280
• Protein ID: WP_083353976.1
• Coordinates: 294474..294788 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1N7CI26
• Locus tag: LIS66_RS01275
• Protein ID: WP_064363398.1
• Coordinates: 294184..294471 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIS66_RS01255 (LIS66_01255)	289958..290362	+	405	WP_042608583.1	S4 domain-containing protein	-
LIS66_RS01260 (LIS66_01260)	290521..291324	-	804	WP_227973447.1	phosphatase PAP2 family protein	-
LIS66_RS01265 (LIS66_01265)	291431..292333	+	903	WP_042608585.1	Hsp33 family molecular chaperone HslO	-
LIS66_RS01270 (LIS66_01270)	292515..294056	+	1542	WP_016772179.1	phosphoenolpyruvate carboxykinase	-
LIS66_RS01275 (LIS66_01275)	294184..294471	-	288	WP_064363398.1	NadS family protein	Antitoxin
LIS66_RS01280 (LIS66_01280)	294474..294788	-	315	WP_083353976.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LIS66_RS01285 (LIS66_01285)	294894..295193	-	300	WP_064363396.1	hypothetical protein	-
LIS66_RS01290 (LIS66_01290)	295539..296681	-	1143	WP_227973448.1	hypothetical protein	-
LIS66_RS01295 (LIS66_01295)	296980..298914	+	1935	WP_227973449.1	ATP-dependent DNA helicase RecQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-73)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11713.42 Da
• Isoelectric Point: 10.0567

>T222226 WP_083353976.1 NZ_CP085885:c294788-294474 [Pseudomonas sp. HN2]
MIFIETSVFTRRVRELLDDDAYATFQKQLVADPSIGDVIEGTGGVRKARVASKGHGKRGGARVIYYHFVSASQIALLMIY
SKNEQQDLTHDERKALKAMIGNWR

Nucleotide

• Length: 315 bp

>T222226 NZ_CP117724:c47594-47307 [Enterobacter asburiae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10727.38 Da
• Isoelectric Point: 8.7453

>AT222226 WP_064363398.1 NZ_CP085885:c294471-294184 [Pseudomonas sp. HN2]
MSKFFDELMESVQEMDEILRGEKKPSREFHVDALQVKQIRKATGMTQAKFAALIDVQLGTLRNWEQGRRQPTGPAKALLK
AIHNDPVHVLSALAD

Nucleotide

• Length: 288 bp

>AT222226 NZ_CP117724:c47833-47594 [Enterobacter asburiae]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1N7CI26