Detailed information of TA system TA222189

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4577457..4578187
• Replicon: chromosome
• Accession: NZ_CP085860
• Organism: Serratia marcescens strain MV-u1-SK1-O

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B4GR63
• Locus tag: LKZ96_RS21810
• Protein ID: WP_004937033.1
• Coordinates: 4577457..4577768 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LKZ96_RS21815
• Protein ID: WP_049208318.1
• Coordinates: 4577765..4578187 (+)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LKZ96_RS21780 (LKZ96_21810)	4572770..4573495	+	726	WP_004937040.1	LPS export ABC transporter ATP-binding protein	-
LKZ96_RS21785 (LKZ96_21815)	4573548..4574981	+	1434	WP_043141961.1	RNA polymerase factor sigma-54	-
LKZ96_RS21790 (LKZ96_21820)	4575005..4575292	+	288	WP_004937038.1	ribosome hibernation promoting factor	-
LKZ96_RS21795 (LKZ96_21825)	4575448..4575909	+	462	WP_016930212.1	PTS IIA-like nitrogen regulatory protein PtsN	-
LKZ96_RS21800 (LKZ96_21830)	4576039..4576893	+	855	WP_015379177.1	RNase adapter RapZ	-
LKZ96_RS21805 (LKZ96_21835)	4576890..4577162	+	273	WP_043141958.1	PTS phosphocarrier protein NPr	-
LKZ96_RS21810 (LKZ96_21840)	4577457..4577768	+	312	WP_004937033.1	type II toxin-antitoxin system HigB family toxin	Toxin
LKZ96_RS21815 (LKZ96_21845)	4577765..4578187	+	423	WP_049208318.1	helix-turn-helix domain-containing protein	Antitoxin
LKZ96_RS21820 (LKZ96_21850)	4578192..4580108	-	1917	WP_049208316.1	PRD domain-containing protein	-
LKZ96_RS21825 (LKZ96_21855)	4580160..4581374	-	1215	WP_089181374.1	lactonase family protein	-
LKZ96_RS21830 (LKZ96_21860)	4581586..4582326	-	741	WP_089181375.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12289.14 Da
• Isoelectric Point: 10.0957

>T222189 WP_004937033.1 NZ_CP085860:4577457-4577768 [Serratia marcescens]
MHIISREPFNNATKQYPNEATALEAIYKALRRGEFRTPDELKALFPSLDRMKYKEKWWVIDVGGNHLRILFFASFETQKV
FIKHIVSHAEYDKLMQHYRRSSS

Nucleotide

• Length: 312 bp

>T222189 NZ_CP117720:293434-293664 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15765.08 Da
• Isoelectric Point: 5.1994

>AT222189 WP_049208318.1 NZ_CP085860:4577765-4578187 [Serratia marcescens]
MITDAIKAADALLRAVPLLQGSHAQQDYREALELVEYLLENDEHHPLIDMLAKKIAEYEDNSAEFTAFNQRIAQLPQGVA
ALRVLMEQHQLSQSDFEHEIGKKSLVNQILNGKRSLTIPHIMALAKRFNLPPALFLPEAD

Nucleotide

• Length: 423 bp

>AT222189 NZ_CP117720:293661-294020 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A8B4GR63