Detailed information of TA system TA222183

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3397365..3398025
• Replicon: chromosome
• Accession: NZ_CP085860
• Organism: Serratia marcescens strain MV-u1-SK1-O

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A656VMD8
• Locus tag: LKZ96_RS16255
• Protein ID: WP_025303493.1
• Coordinates: 3397672..3398025 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A8G2G5Z5
• Locus tag: LKZ96_RS16250
• Protein ID: WP_016927038.1
• Coordinates: 3397365..3397667 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LKZ96_RS16230 (LKZ96_16250)	3392842..3393756	-	915	WP_004928592.1	branched-chain amino acid ABC transporter permease	-
LKZ96_RS16235 (LKZ96_16255)	3393882..3395000	-	1119	WP_016927040.1	branched-chain amino acid ABC transporter substrate-binding protein	-
LKZ96_RS16245 (LKZ96_16265)	3395732..3396940	-	1209	WP_213928418.1	aldose 1-epimerase family protein	-
LKZ96_RS16250 (LKZ96_16270)	3397365..3397667	-	303	WP_016927038.1	XRE family transcriptional regulator	Antitoxin
LKZ96_RS16255 (LKZ96_16275)	3397672..3398025	-	354	WP_025303493.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LKZ96_RS16260 (LKZ96_16280)	3398464..3399759	+	1296	WP_025303494.1	MFS transporter	-
LKZ96_RS16265 (LKZ96_16285)	3399786..3400592	+	807	WP_213928417.1	substrate-binding domain-containing protein	-
LKZ96_RS16270 (LKZ96_16290)	3400567..3401466	-	900	WP_197822133.1	LysR family transcriptional regulator	-
LKZ96_RS16275 (LKZ96_16295)	3401570..3401935	-	366	WP_004935528.1	diacylglycerol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-89)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13548.47 Da
• Isoelectric Point: 9.0170

>T222183 WP_025303493.1 NZ_CP085860:c3398025-3397672 [Serratia marcescens]
VWEIKTTDAFDHWYRSLSDADRAGVLAALMVLREKGPGLPRPYADTVKGSRYSNMKELRIQCRGEPLRAFFAFDPYRIGI
VLCAGNKAGNEKRFYDRMIQVADREFTNWLNTLNEKE

Nucleotide

• Length: 354 bp

>T222183 NZ_CP117717:3895928-3896206 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11093.96 Da
• Isoelectric Point: 6.4738

>AT222183 WP_016927038.1 NZ_CP085860:c3397667-3397365 [Serratia marcescens]
MGRTLEQLIADEKPEVVAKAQALAADIMLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLRLDIELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT222183 NZ_CP117717:3895665-3895925 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A656VMD8