Detailed information of TA system TA222141

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1398066..1398437
• Replicon: chromosome
• Accession: NZ_CP085842
• Organism: Escherichia coli strain B95. delta A

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: LJ838_RS06980
• Protein ID: WP_001317028.1
• Coordinates: 1398243..1398437 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1398066..1398244 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJ838_RS06955 (1394021)	1394021..1395394	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
LJ838_RS06960 (1395523)	1395523..1396458	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
LJ838_RS06965 (1396510)	1396510..1397745	-	1236	WP_000040858.1	site-specific integrase	-
LJ838_RS06970 (1397747)	1397747..1397962	-	216	WP_000079604.1	excisionase XisR	-
- (1398066)	1398066..1398244	+	179	NuclAT_0	-	Antitoxin
- (1398066)	1398066..1398244	+	179	NuclAT_0	-	Antitoxin
- (1398066)	1398066..1398244	+	179	NuclAT_0	-	Antitoxin
- (1398066)	1398066..1398244	+	179	NuclAT_0	-	Antitoxin
LJ838_RS06975 (1398041)	1398041..1398250	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
LJ838_RS06980 (1398243)	1398243..1398437	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
LJ838_RS06985 (1398494)	1398494..1399303	-	810	WP_000166319.1	recombination protein RecT	-
LJ838_RS06990 (1399296)	1399296..1400395	-	1100	Protein_1363	PD-(D/E)XK nuclease-like domain-containing protein	-
LJ838_RS06995 (1400497)	1400497..1400772	-	276	WP_000632297.1	protein RacC	-
LJ838_RS07000 (1400847)	1400847..1401017	-	171	WP_001352098.1	YdaE family protein	-
LJ838_RS07005 (1401017)	1401017..1401238	-	222	WP_000560223.1	killing protein KilR	-
LJ838_RS07010 (1401680)	1401680..1402168	+	489	WP_001312793.1	superinfection exclusion B family protein	-
LJ838_RS07015 (1402165)	1402165..1402320	-	156	WP_001169151.1	YdaF family protein	-
LJ838_RS22180 (1402331)	1402331..1402465	-	135	WP_000233810.1	hypothetical protein	-
LJ838_RS07020 (1402464)	1402464..1402676	+	213	WP_012775985.1	phage lysis protein EssD	-
LJ838_RS07025 (1402681)	1402681..1403025	+	345	WP_000193293.1	YdfR family protein	-
LJ838_RS07030 (1402991)	1402991..1403263	-	273	WP_000370546.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1387765..1408575	20810

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T222141 WP_001317028.1 NZ_CP085842:c1398437-1398243 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T222141 NZ_CP117709:2998170-2998430 [Streptomyces sp. MMBL 11-1]
GTGAAGTTCGTGTGGGACCAGTCGTCGTGGGACGACTACGTCTGGTGGCAGAACCAGGACCGTAAGATCCTCAAGAGGAT
CAACACACTGCTCCAGGACATCGCCCGGAACGGCAACGAAGGAATGGGCAAACCGGAACCGTTGAAGCACGGTTTTCACG
GGTACTGGTCCCGCCGCATCACCGACGAGCACCGGCTCATCTACAAAGTGGCCGACGATGAGGTCCGTGTCGCCGCCTGC
CGCTACCACTACGGTCACTGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT222141 NZ_CP085842:1398066-1398244 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin