Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 250533..251074 Replicon chromosome
Accession NZ_CP085842
Organism Escherichia coli strain B95. delta A

Toxin (Protein)


Gene name yafQ Uniprot ID A0A140NAP5
Locus tag LJ838_RS01150 Protein ID WP_000615981.1
Coordinates 250533..250811 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag LJ838_RS01155 Protein ID WP_000729704.1
Coordinates 250814..251074 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJ838_RS01135 (248115) 248115..248693 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
LJ838_RS01140 (248899) 248899..249666 + 768 WP_000333379.1 class II glutamine amidotransferase -
LJ838_RS01145 (249637) 249637..250377 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LJ838_RS01150 (250533) 250533..250811 - 279 WP_000615981.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LJ838_RS01155 (250814) 250814..251074 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LJ838_RS01160 (251260) 251260..252033 + 774 WP_001353765.1 C40 family peptidase -
LJ838_RS01165 (252209) 252209..252706 + 498 WP_000006260.1 REP-associated tyrosine transposase RayT -
LJ838_RS01170 (252922) 252922..254634 - 1713 Protein_226 flagellar biosynthesis protein FlhA -
LJ838_RS01175 (254606) 254606..255391 + 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10860.65 Da        Isoelectric Point: 10.0702

>T222135 WP_000615981.1 NZ_CP085842:c250811-250533 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T222135 NZ_CP117698:c4071389-4071105 [Salmonella enterica subsp. enterica serovar London]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGAAGTTGTGATTGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT222135 WP_000729704.1 NZ_CP085842:c251074-250814 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT222135 NZ_CP117698:c4071621-4071379 [Salmonella enterica subsp. enterica serovar London]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MMG
PDB 4MMJ
PDB 4ML2
PDB 4ML0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References