Detailed information of TA system TA222081

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 160673..161275
• Replicon: chromosome
• Accession: NZ_CP085824
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain Ivysaur

Toxin (Protein)

• Gene name: higB
• Uniprot ID: M7S4R6
• Locus tag: LJF36_RS00750
• Protein ID: WP_001159635.1
• Coordinates: 160964..161275 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LJF36_RS00745
• Protein ID: WP_000362050.1
• Coordinates: 160673..160963 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF36_RS00730 (158166)	158166..159068	+	903	WP_000331364.1	formate dehydrogenase subunit beta	-
LJF36_RS00735 (159065)	159065..159700	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
LJF36_RS00740 (159697)	159697..160626	+	930	WP_000027736.1	formate dehydrogenase accessory protein FdhE	-
LJF36_RS00745 (160673)	160673..160963	-	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
LJF36_RS00750 (160964)	160964..161275	-	312	WP_001159635.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
LJF36_RS00755 (161493)	161493..162422	+	930	WP_001127705.1	alpha/beta hydrolase	-
LJF36_RS00760 (162508)	162508..162819	+	312	WP_000558168.1	type II toxin-antitoxin system HigB family toxin	-
LJF36_RS00765 (162816)	162816..163262	+	447	WP_001259009.1	type II toxin-antitoxin system HigA family antitoxin	-
LJF36_RS00770 (163277)	163277..164218	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
LJF36_RS00775 (164263)	164263..164700	-	438	WP_000560968.1	D-aminoacyl-tRNA deacylase	-
LJF36_RS00780 (164697)	164697..165569	-	873	WP_000921423.1	virulence factor BrkB family protein	-
LJF36_RS00785 (165563)	165563..166162	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12340.30 Da
• Isoelectric Point: 9.4460

>T222081 WP_001159635.1 NZ_CP085824:c161275-160964 [Salmonella enterica subsp. enterica serovar Enteritidis]
MQFIETELFTEDVKKLLDEDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T222081 NZ_CP117672:c4653617-4653306 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT222081 WP_000362050.1 NZ_CP085824:c160963-160673 [Salmonella enterica subsp. enterica serovar Enteritidis]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT222081 NZ_CP117672:c4653305-4653015 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A3V2JK97