Detailed information of TA system TA222027

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4555579..4556333
• Replicon: chromosome
• Accession: NZ_CP085821
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain Charizard

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5RFE2
• Locus tag: LJF63_RS22245
• Protein ID: WP_000558168.1
• Coordinates: 4556022..4556333 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LJF63_RS22240
• Protein ID: WP_001259009.1
• Coordinates: 4555579..4556025 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF63_RS22210 (4550755)	4550755..4551651	+	897	WP_001520529.1	sugar kinase	-
LJF63_RS22215 (4551685)	4551685..4552488	+	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
LJF63_RS22220 (4552679)	4552679..4553278	+	600	WP_000965695.1	glucose-1-phosphatase	-
LJF63_RS22225 (4553272)	4553272..4554144	+	873	WP_000921423.1	virulence factor BrkB family protein	-
LJF63_RS22230 (4554141)	4554141..4554578	+	438	WP_000560968.1	D-aminoacyl-tRNA deacylase	-
LJF63_RS22235 (4554623)	4554623..4555564	+	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
LJF63_RS22240 (4555579)	4555579..4556025	-	447	WP_001259009.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
LJF63_RS22245 (4556022)	4556022..4556333	-	312	WP_000558168.1	type II toxin-antitoxin system HigB family toxin	Toxin
LJF63_RS22250 (4556419)	4556419..4557348	-	930	WP_001127705.1	alpha/beta hydrolase	-
LJF63_RS22255 (4557566)	4557566..4557877	+	312	WP_001159635.1	cytotoxic translational repressor of toxin-antitoxin stability system	-
LJF63_RS22260 (4557878)	4557878..4558168	+	291	WP_000362050.1	DNA-binding transcriptional regulator	-
LJF63_RS22265 (4558215)	4558215..4559144	-	930	WP_000027736.1	formate dehydrogenase accessory protein FdhE	-
LJF63_RS22270 (4559141)	4559141..4559776	-	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
LJF63_RS22275 (4559773)	4559773..4560675	-	903	WP_000331364.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12324.26 Da
• Isoelectric Point: 9.3143

>T222027 WP_000558168.1 NZ_CP085821:c4556333-4556022 [Salmonella enterica subsp. enterica serovar Enteritidis]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTLTQPEEMKRYIPSLDNFKHRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYDRGNKE

Nucleotide

• Length: 312 bp

>T222027 NZ_CP117659:2048189-2048291 [Escherichia albertii]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAAAACA
GGAATCGTTTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16720.06 Da
• Isoelectric Point: 6.6451

>AT222027 WP_001259009.1 NZ_CP085821:c4556025-4555579 [Salmonella enterica subsp. enterica serovar Enteritidis]
MRTHRQMDATSAKKIVDTFSDAVKSVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT222027 NZ_CP117659:c2048293-2048061 [Escherichia albertii]
ATAAAAAGAGACCGAAAACGATTCCTGTTTTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCGTACAAAATGGTCAG
TAAAAAGCGCGTCGGCCATCGTCTTAAATGTTAAAAACCGCCCGTTCTGGTGAAAGAACTGTGGCGGTTTTTT

Structures

Toxin

• AlphaFold DB: A0A1R2S541