Detailed information of TA system TA221963

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1697510..1698112
• Replicon: chromosome
• Accession: NZ_CP085819
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain Blastoise

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C0Q3J8
• Locus tag: LJF65_RS07945
• Protein ID: WP_001159630.1
• Coordinates: 1697801..1698112 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LJF65_RS07940
• Protein ID: WP_000362050.1
• Coordinates: 1697510..1697800 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF65_RS07925 (1695003)	1695003..1695905	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
LJF65_RS07930 (1695902)	1695902..1696537	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
LJF65_RS07935 (1696534)	1696534..1697463	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
LJF65_RS07940 (1697510)	1697510..1697800	-	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
LJF65_RS07945 (1697801)	1697801..1698112	-	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
LJF65_RS07950 (1698330)	1698330..1699259	+	930	WP_001127703.1	alpha/beta hydrolase	-
LJF65_RS07955 (1699345)	1699345..1699656	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
LJF65_RS07960 (1699653)	1699653..1700099	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
LJF65_RS07965 (1700114)	1700114..1701055	-	942	WP_227761035.1	fatty acid biosynthesis protein FabY	-
LJF65_RS07970 (1701100)	1701100..1701537	-	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
LJF65_RS07975 (1701534)	1701534..1702406	-	873	WP_000921427.1	virulence factor BrkB family protein	-
LJF65_RS07980 (1702400)	1702400..1702999	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12326.27 Da
• Isoelectric Point: 9.4460

>T221963 WP_001159630.1 NZ_CP085819:c1698112-1697801 [Salmonella enterica subsp. enterica serovar Typhimurium]
MQFIETELFTEDVKKLLDDDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T221963 NZ_CP117644:c79806-79408 [Escherichia albertii]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATTCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT221963 WP_000362050.1 NZ_CP085819:c1697800-1697510 [Salmonella enterica subsp. enterica serovar Typhimurium]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT221963 NZ_CP117644:c80033-79806 [Escherichia albertii]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A0R9NS64