Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3562530..3563200 Replicon chromosome
Accession NC_009525
Organism Mycobacterium tuberculosis H37Ra

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag MRA_RS16910 Protein ID WP_003899954.1
Coordinates 3562530..3562874 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag MRA_RS16915 Protein ID WP_003899955.1
Coordinates 3562871..3563200 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MRA_RS16880 3557603..3558463 + 861 WP_003416628.1 alpha/beta hydrolase -
MRA_RS16885 3558438..3558953 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
MRA_RS16890 3559193..3559486 + 294 WP_003416635.1 hypothetical protein -
MRA_RS16895 3559774..3561063 + 1290 WP_003416640.1 ATP-binding protein -
MRA_RS16900 3561410..3561844 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
MRA_RS16905 3561847..3562299 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
MRA_RS16910 3562530..3562874 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MRA_RS16915 3562871..3563200 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
MRA_RS16925 3563562..3564823 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
MRA_RS16930 3565096..3565443 + 348 WP_003899956.1 DUF2384 domain-containing protein -
MRA_RS16935 3565440..3566060 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
MRA_RS16940 3566220..3567485 - 1266 WP_003912135.1 hypothetical protein -
MRA_RS16945 3567653..3567862 + 210 WP_003416778.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T22188 WP_003899954.1 NC_009525:3562530-3562874 [Mycobacterium tuberculosis H37Ra]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T22188 NC_009525:3562530-3562874 [Mycobacterium tuberculosis H37Ra]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT22188 WP_003899955.1 NC_009525:3562871-3563200 [Mycobacterium tuberculosis H37Ra]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT22188 NC_009525:3562871-3563200 [Mycobacterium tuberculosis H37Ra]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References