Detailed information of TA system TA221830

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 659645..660399
• Replicon: chromosome
• Accession: NZ_CP085814
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain Plusle

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5RFE2
• Locus tag: LJF38_RS03145
• Protein ID: WP_000558168.1
• Coordinates: 660088..660399 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LJF38_RS03140
• Protein ID: WP_001259009.1
• Coordinates: 659645..660091 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF38_RS03110 (654821)	654821..655717	+	897	WP_001520529.1	sugar kinase	-
LJF38_RS03115 (655751)	655751..656554	+	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
LJF38_RS03120 (656745)	656745..657344	+	600	WP_000965695.1	glucose-1-phosphatase	-
LJF38_RS03125 (657338)	657338..658210	+	873	WP_000921423.1	virulence factor BrkB family protein	-
LJF38_RS03130 (658207)	658207..658644	+	438	WP_000560968.1	D-aminoacyl-tRNA deacylase	-
LJF38_RS03135 (658689)	658689..659630	+	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
LJF38_RS03140 (659645)	659645..660091	-	447	WP_001259009.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
LJF38_RS03145 (660088)	660088..660399	-	312	WP_000558168.1	type II toxin-antitoxin system HigB family toxin	Toxin
LJF38_RS03150 (660485)	660485..661414	-	930	WP_001127705.1	alpha/beta hydrolase	-
LJF38_RS03155 (661632)	661632..661943	+	312	WP_001159635.1	cytotoxic translational repressor of toxin-antitoxin stability system	-
LJF38_RS03160 (661944)	661944..662234	+	291	WP_000362050.1	DNA-binding transcriptional regulator	-
LJF38_RS03165 (662281)	662281..663210	-	930	WP_000027736.1	formate dehydrogenase accessory protein FdhE	-
LJF38_RS03170 (663207)	663207..663842	-	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
LJF38_RS03175 (663839)	663839..664741	-	903	WP_000331364.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12324.26 Da
• Isoelectric Point: 9.3143

>T221830 WP_000558168.1 NZ_CP085814:c660399-660088 [Salmonella enterica subsp. enterica serovar Enteritidis]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTLTQPEEMKRYIPSLDNFKHRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYDRGNKE

Nucleotide

• Length: 312 bp

>T221830 NZ_CP117601:c67846-67568 [Escherichia albertii]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGAACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTGTAT
ATTCTGCGAGTGTTGCATACAGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16720.06 Da
• Isoelectric Point: 6.6451

>AT221830 WP_001259009.1 NZ_CP085814:c660091-659645 [Salmonella enterica subsp. enterica serovar Enteritidis]
MRTHRQMDATSAKKIVDTFSDAVKSVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT221830 NZ_CP117601:c68115-67846 [Escherichia albertii]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A1R2S541