Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2988554..2989202 Replicon chromosome
Accession NC_009525
Organism Mycobacterium tuberculosis H37Ra

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag MRA_RS14155 Protein ID WP_003899414.1
Coordinates 2988554..2988877 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag MRA_RS14160 Protein ID WP_003899415.1
Coordinates 2988957..2989202 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MRA_RS14135 2984128..2985389 + 1262 WP_143711313.1 IS3-like element IS987 family transposase -
MRA_RS22775 2985416..2985601 - 186 WP_009935348.1 hypothetical protein -
MRA_RS14140 2985763..2987202 - 1440 WP_009935349.1 phage major capsid protein -
MRA_RS14145 2987210..2987743 - 534 WP_003899412.1 HK97 family phage prohead protease -
MRA_RS21765 2987896..2988387 - 492 WP_003900541.1 phage terminase small subunit P27 family -
MRA_RS14155 2988554..2988877 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
MRA_RS14160 2988957..2989202 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
MRA_RS14165 2989199..2990626 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
MRA_RS14170 2990628..2991020 - 393 WP_003899417.1 DUF2742 domain-containing protein -
MRA_RS14175 2991017..2991277 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
MRA_RS14180 2991294..2991656 - 363 WP_003900543.1 hypothetical protein -
MRA_RS14185 2991659..2992786 - 1128 WP_003899420.1 site-specific integrase -
MRA_RS14190 2992931..2993158 - 228 WP_003899421.1 hypothetical protein -
MRA_RS21770 2993155..2993544 - 390 WP_003899422.1 hypothetical protein -
MRA_RS14195 2993450..2993722 + 273 WP_003900544.1 hypothetical protein -
MRA_RS14200 2993821..2994054 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2982519..2992786 10267


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-69)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T22180 WP_003899414.1 NC_009525:c2988877-2988554 [Mycobacterium tuberculosis H37Ra]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T22180 NC_009525:c2988877-2988554 [Mycobacterium tuberculosis H37Ra]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT22180 WP_003899415.1 NC_009525:c2989202-2988957 [Mycobacterium tuberculosis H37Ra]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT22180 NC_009525:c2989202-2988957 [Mycobacterium tuberculosis H37Ra]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References