Detailed information of TA system TA221692

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4598842..4599596
• Replicon: chromosome
• Accession: NZ_CP085806
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain Marill

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5RFE2
• Locus tag: LJF46_RS22445
• Protein ID: WP_000558168.1
• Coordinates: 4599285..4599596 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LJF46_RS22440
• Protein ID: WP_001259009.1
• Coordinates: 4598842..4599288 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF46_RS22410 (4594018)	4594018..4594914	+	897	WP_001520529.1	sugar kinase	-
LJF46_RS22415 (4594948)	4594948..4595751	+	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
LJF46_RS22420 (4595942)	4595942..4596541	+	600	WP_000965695.1	glucose-1-phosphatase	-
LJF46_RS22425 (4596535)	4596535..4597407	+	873	WP_000921423.1	virulence factor BrkB family protein	-
LJF46_RS22430 (4597404)	4597404..4597841	+	438	WP_000560968.1	D-aminoacyl-tRNA deacylase	-
LJF46_RS22435 (4597886)	4597886..4598827	+	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
LJF46_RS22440 (4598842)	4598842..4599288	-	447	WP_001259009.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
LJF46_RS22445 (4599285)	4599285..4599596	-	312	WP_000558168.1	type II toxin-antitoxin system HigB family toxin	Toxin
LJF46_RS22450 (4599682)	4599682..4600611	-	930	WP_001127705.1	alpha/beta hydrolase	-
LJF46_RS22455 (4600829)	4600829..4601140	+	312	WP_001159635.1	cytotoxic translational repressor of toxin-antitoxin stability system	-
LJF46_RS22460 (4601141)	4601141..4601431	+	291	WP_000362050.1	DNA-binding transcriptional regulator	-
LJF46_RS22465 (4601478)	4601478..4602407	-	930	WP_000027736.1	formate dehydrogenase accessory protein FdhE	-
LJF46_RS22470 (4602404)	4602404..4603039	-	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
LJF46_RS22475 (4603036)	4603036..4603938	-	903	WP_000331364.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12324.26 Da
• Isoelectric Point: 9.3143

>T221692 WP_000558168.1 NZ_CP085806:c4599596-4599285 [Salmonella enterica subsp. enterica serovar Enteritidis]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTLTQPEEMKRYIPSLDNFKHRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYDRGNKE

Nucleotide

• Length: 312 bp

>T221692 NZ_CP117568:c3683791-3683450 [Escherichia albertii]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTAAATTAACCGTAAGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCACCCAACCACCCGCCACCGAAGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAAGCAGGTGCAGGAGTTTATTGGGGTAATTAC
AGGTAAACAGAAAGTCGCCTGA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16720.06 Da
• Isoelectric Point: 6.6451

>AT221692 WP_001259009.1 NZ_CP085806:c4599288-4598842 [Salmonella enterica subsp. enterica serovar Enteritidis]
MRTHRQMDATSAKKIVDTFSDAVKSVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT221692 NZ_CP117568:3683786-3683862 [Escherichia albertii]
AGTCATAACTGCTATTCTCCGATAATAGTGATTGTGATGAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A1R2S541