Detailed information of TA system TA221531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3357168..3357922
• Replicon: chromosome
• Accession: NZ_CP085800
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain Taillow

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5RFE2
• Locus tag: LJF53_RS16495
• Protein ID: WP_000558168.1
• Coordinates: 3357168..3357479 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LJF53_RS16500
• Protein ID: WP_001259009.1
• Coordinates: 3357476..3357922 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF53_RS16460 (3352505)	3352505..3352813	+	309	Protein_3209	formate dehydrogenase	-
LJF53_RS16465 (3352826)	3352826..3353728	+	903	WP_000331364.1	formate dehydrogenase subunit beta	-
LJF53_RS16470 (3353725)	3353725..3354360	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
LJF53_RS16475 (3354357)	3354357..3355286	+	930	WP_000027736.1	formate dehydrogenase accessory protein FdhE	-
LJF53_RS16480 (3355333)	3355333..3355623	-	291	WP_000362050.1	DNA-binding transcriptional regulator	-
LJF53_RS16485 (3355624)	3355624..3355935	-	312	WP_001159635.1	cytotoxic translational repressor of toxin-antitoxin stability system	-
LJF53_RS16490 (3356153)	3356153..3357082	+	930	WP_001127705.1	alpha/beta hydrolase	-
LJF53_RS16495 (3357168)	3357168..3357479	+	312	WP_000558168.1	type II toxin-antitoxin system HigB family toxin	Toxin
LJF53_RS16500 (3357476)	3357476..3357922	+	447	WP_001259009.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
LJF53_RS16505 (3357937)	3357937..3358878	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
LJF53_RS16510 (3358923)	3358923..3359360	-	438	WP_000560968.1	D-aminoacyl-tRNA deacylase	-
LJF53_RS16515 (3359357)	3359357..3360229	-	873	WP_000921423.1	virulence factor BrkB family protein	-
LJF53_RS16520 (3360223)	3360223..3360822	-	600	WP_000965695.1	glucose-1-phosphatase	-
LJF53_RS16525 (3361013)	3361013..3361816	-	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
LJF53_RS16530 (3361850)	3361850..3362746	-	897	WP_001520529.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12324.26 Da
• Isoelectric Point: 9.3143

>T221531 WP_000558168.1 NZ_CP085800:3357168-3357479 [Salmonella enterica subsp. enterica serovar Enteritidis]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTLTQPEEMKRYIPSLDNFKHRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYDRGNKE

Nucleotide

• Length: 312 bp

>T221531 NZ_CP117405:c89759-88875 [Lactococcus lactis]
ATGAATTCAAATCGTTTAAAAGATCTGATAAAAAATCAAGATACAAGTATTAGTTCTGAAGAATATAGAATTAGATATGC
GACTGAAAGATTTTTACTCAGAGTTCAAAGAAGTAGTTATAGAGACAAGCTTATTTTAAAGGGTGGCTTTTTGTTAGGCA
CACTTTTTAAAGTCGAGCAAAGAACAACAAAAGATTTAGATACACTAATAACGGAATTGAGTGTGAGCAGAGAACAGGTA
GAAACAATGCTTGAATCAATTATTCAAATTGACTTAAACGATAATGTAAAATTTGAGCTTATAAAACTAATTGATTCCCA
GCAACGAAGAATTTATGATGGGTTTAAAGCTAAACTCAAAATGAACTTCTTAGACGAGCGAACATTTGTCAACTTTGATT
TAGATCTAGGAGTTGGTGATACTATTACACCAGAGGCTAAAAAAATTAATATCCCTTTATTATTTAATGAAGTAAAGGGT
GAAAAAACGGAGATTGCATTACTAGCATATCCTTTGGAAACAATATTAGCAGAAAAGACCGAAATAATTTTAAATTTAGG
AACAAATAATTCTAGAATGAAAGATTTTTATGACATGCATTTGTTATTAAATGATCCTCGAAAACCAGATAATGAGTTAC
TTTACCAAGCTTTTGAGAATACTTGGATATTCAGACACCCCAATCTTTCAATAGACAATGAGAGATTTGAAGATTGGTAT
TATGTTATAGAGTCTATTTTAGAAAATGATAAAATGAATAGTATTTCTTGGCATAATTATATAAAAGATCGACTGTATGC
TAAAGACTTAAATTTAAAGAATATAGTCTTGCAGTTTAAACGTTATTTAGAAGAGTTAGAAAAAGTATATATTAAGCGTA
AATAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16720.06 Da
• Isoelectric Point: 6.6451

>AT221531 WP_001259009.1 NZ_CP085800:3357476-3357922 [Salmonella enterica subsp. enterica serovar Enteritidis]
MRTHRQMDATSAKKIVDTFSDAVKSVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT221531 NZ_CP117405:c90650-89781 [Lactococcus lactis]
ATGTATTCAACTATATATGACGTTTTTATAAATAATATTGATAAAAGCGAAGAAGAAAAAAAGAATATGATTCACGAAAT
GGTTGATATTATGGATATTGATCAATTATCAAAAGTTATTGATTTTCTTAAAGAACCTTATTTTTTAAAAAAACTAAAAC
CATATTTGGTGAATGATATGCTTCCTCCCATTGAATCTCCTGAATTTTTATTTCTTTTACAATTAGCGAAATACAATGGA
AACTTAGTTAAAAAATTGGCTCGTGAAAATAAATTGAGTGACTATTATATTGATAAGTTTATAAATAAATATCGTTTGAG
AGAAGTTTCAAAAAGTATTTATGTATTTCCGGGAAATGAAATAGATGGAAATTTTGTGTTTCAAGCACAATATACTCGCT
CTGTAATCTCTCATGAAACAGCGTTATACATGCATGATTTAAGTGATGTTATTCCTAGAAAAACTATAATGAGTATGCCG
ATTTCTTACAAATTATCACAAGTAGAAAAAAACGAGAGAAAATATATAAAAGTACATAAGGAAGTTCTTGGTAGTAATCG
GACATATTATGTAGTTGAATATTATCAAAATGATCCTATTTATTTGATGAAAAATCAACCGATAGGAAATTCTCAAATTC
AACTAATGAATACTCAGCAAGGCAATGAAGTGAGAGTTACCTCAGTTGAGCGCACTATTGCTGATATCTTTAAACCAAGT
TCTAATGTTGAAGAGGAAGTTAAGGAAACAGCTTTAGAGCAGTATTCTAAAAGAGGAAACTACGATAAGAGTAGACTATT
GCGGATTTCTAAACAACAAAATGTTGAAAAAGAGATTGAACATACTCTTTGGAAGTTGAAGTTGCATTAA

Structures

Toxin

• AlphaFold DB: A0A1R2S541