Detailed information of TA system TA221389

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1361855..1362457
• Replicon: chromosome
• Accession: NZ_CP085795
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain Frogadier

Toxin (Protein)

• Gene name: higB
• Uniprot ID: M7S4R6
• Locus tag: LJF59_RS06520
• Protein ID: WP_001159635.1
• Coordinates: 1361855..1362166 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LJF59_RS06525
• Protein ID: WP_000362050.1
• Coordinates: 1362167..1362457 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF59_RS06485 (1356968)	1356968..1357567	+	600	WP_000965695.1	glucose-1-phosphatase	-
LJF59_RS06490 (1357561)	1357561..1358433	+	873	WP_000921423.1	virulence factor BrkB family protein	-
LJF59_RS06495 (1358430)	1358430..1358867	+	438	WP_000560968.1	D-aminoacyl-tRNA deacylase	-
LJF59_RS06500 (1358912)	1358912..1359853	+	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
LJF59_RS06505 (1359868)	1359868..1360314	-	447	WP_001259009.1	type II toxin-antitoxin system HigA family antitoxin	-
LJF59_RS06510 (1360311)	1360311..1360622	-	312	WP_000558168.1	type II toxin-antitoxin system HigB family toxin	-
LJF59_RS06515 (1360708)	1360708..1361637	-	930	WP_001127705.1	alpha/beta hydrolase	-
LJF59_RS06520 (1361855)	1361855..1362166	+	312	WP_001159635.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
LJF59_RS06525 (1362167)	1362167..1362457	+	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
LJF59_RS06530 (1362504)	1362504..1363433	-	930	WP_000027736.1	formate dehydrogenase accessory protein FdhE	-
LJF59_RS06535 (1363430)	1363430..1364065	-	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
LJF59_RS06540 (1364062)	1364062..1364964	-	903	WP_000331364.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12340.30 Da
• Isoelectric Point: 9.4460

>T221389 WP_001159635.1 NZ_CP085795:1361855-1362166 [Salmonella enterica subsp. enterica serovar Enteritidis]
MQFIETELFTEDVKKLLDEDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T221389 NZ_CP117386:3486794-3487012 [Salmonella enterica subsp. enterica serovar Uganda]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACAATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT221389 WP_000362050.1 NZ_CP085795:1362167-1362457 [Salmonella enterica subsp. enterica serovar Enteritidis]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT221389 NZ_CP117386:3486392-3486766 [Salmonella enterica subsp. enterica serovar Uganda]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAAC
ATATTGCAACTTTCGCTCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A3V2JK97