Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 334415..335040 Replicon chromosome
Accession NZ_CP085794
Organism Salmonella enterica subsp. enterica serovar Enteritidis strain Greninja

Toxin (Protein)


Gene name vapC Uniprot ID M7SD17
Locus tag LJF60_RS01545 Protein ID WP_000911336.1
Coordinates 334415..334813 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID M7S3S8
Locus tag LJF60_RS01550 Protein ID WP_000557549.1
Coordinates 334813..335040 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF60_RS01530 (331092) 331092..331673 - 582 WP_001244651.1 fimbrial protein -
LJF60_RS01535 (332392) 332392..333024 - 633 WP_000835265.1 YfdX family protein -
LJF60_RS01540 (333071) 333071..333607 - 537 WP_001038502.1 STM3031 family outer membrane protein -
LJF60_RS01545 (334415) 334415..334813 - 399 WP_000911336.1 tRNA(fMet)-specific endonuclease VapC Toxin
LJF60_RS01550 (334813) 334813..335040 - 228 WP_000557549.1 toxin-antitoxin system antitoxin VapB Antitoxin
LJF60_RS01555 (335249) 335249..335500 + 252 WP_001576352.1 hypothetical protein -
LJF60_RS01560 (335780) 335780..336586 + 807 WP_001574939.1 DUF1460 domain-containing protein -
LJF60_RS01570 (336871) 336871..337629 - 759 WP_000244328.1 amidase activator ActS -
LJF60_RS01575 (337894) 337894..338439 + 546 WP_000133994.1 isopentenyl-diphosphate Delta-isomerase -
LJF60_RS01580 (338515) 338515..340032 - 1518 WP_000003339.1 lysine--tRNA ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 325976..336586 10610


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-123)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14938.30 Da        Isoelectric Point: 7.2155

>T221357 WP_000911336.1 NZ_CP085794:c334813-334415 [Salmonella enterica subsp. enterica serovar Enteritidis]
MLKFMLDTNTCIFTIKNKPEHIRERFNLNTSRMCISSITLMELIYGAEKSLAPERNLAVVEGFISRLEVLDYDTQAAIHT
GQIRAELARKGTPVGPYDQMIAGHAGSRGLVVVTNNLREFERIPGIRIEDWC

Download         Length: 399 bp

>T221357 NZ_CP117382:3487063-3487281 [Salmonella enterica subsp. enterica serovar Uganda]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACAATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8514.51 Da        Isoelectric Point: 5.0210

>AT221357 WP_000557549.1 NZ_CP085794:c335040-334813 [Salmonella enterica subsp. enterica serovar Enteritidis]
MHTTLFFSNRTQAVRLPKSISFPEDVKHVEIIAVGRSRIITPVGESWDSWFDGEGASTDFMSTREQPAVQEREGF

Download         Length: 228 bp

>AT221357 NZ_CP117382:3486661-3487035 [Salmonella enterica subsp. enterica serovar Uganda]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAAC
ATATTGCAACTTTCGCTCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6IFC
PDB 6IFM

Antitoxin

Source ID Structure
PDB 6IFM
AlphaFold DB A0A3V2Y5V5

References