Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4185200..4185875 Replicon chromosome
Accession NZ_CP085774
Organism Enterobacter hormaechei strain 48947CZ

Toxin (Protein)


Gene name higB Uniprot ID A0A837FDC1
Locus tag LJU36_RS20235 Protein ID WP_015571638.1
Coordinates 4185200..4185499 (+) Length 100 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2J0PXC9
Locus tag LJU36_RS20240 Protein ID WP_015571639.1
Coordinates 4185510..4185875 (+) Length 122 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJU36_RS20225 (LJU36_20225) 4181577..4183769 + 2193 WP_017692801.1 type I secretion system permease/ATPase -
LJU36_RS20230 (LJU36_20230) 4183750..4184949 + 1200 WP_017382757.1 HlyD family efflux transporter periplasmic adaptor subunit -
LJU36_RS20235 (LJU36_20235) 4185200..4185499 + 300 WP_015571638.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LJU36_RS20240 (LJU36_20240) 4185510..4185875 + 366 WP_015571639.1 HigA family addiction module antitoxin Antitoxin
LJU36_RS20245 (LJU36_20245) 4185902..4187083 - 1182 WP_015571640.1 PLP-dependent aminotransferase family protein -
LJU36_RS20250 (LJU36_20250) 4187104..4187991 - 888 WP_015571641.1 LysR family transcriptional regulator -
LJU36_RS20255 (LJU36_20255) 4188089..4188691 + 603 WP_015571642.1 short chain dehydrogenase -
LJU36_RS20260 (LJU36_20260) 4188685..4189419 - 735 WP_017692800.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(25-72)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11624.29 Da        Isoelectric Point: 9.6776

>T221280 WP_015571638.1 NZ_CP085774:4185200-4185499 [Enterobacter hormaechei]
MINHFRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Download         Length: 300 bp

>T221280 NZ_CP085774:4185200-4185499 [Enterobacter hormaechei]
ATGATTAATCATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTCTACGGGAGATCGAGCAATGTTATTCCTTTGAATCT
GGAAACAGCGCTTGCAAGAAAGCTCGACATCATCAATGCGGCCATGTCGCACCTGGATTTACGATCGCCACCGGGCAATA
TGTATGAAGCATTGAGTCCTCCGTTGAAGGGATATTCCTCTATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
TCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin


Download         Length: 122 a.a.        Molecular weight: 13705.73 Da        Isoelectric Point: 9.5936

>AT221280 WP_015571639.1 NZ_CP085774:4185510-4185875 [Enterobacter hormaechei]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Download         Length: 366 bp

>AT221280 NZ_CP085774:4185510-4185875 [Enterobacter hormaechei]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGAGGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAACGATCTGGCAGAGATGCTAAATGTACACCGCAATACCGTAAGCGCGTTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTAATACCACTATTGAATTTTGGCTGAACTTACAGCTAAACGTTGAT
ATCTGGGAAGCGCAATCTAACTCCCGCACGCAGGAGGAGTTAAGCCGGATAAAAACCGTTGCGGAAGTCATGGCGAAGCG
AAAATCCGGCAAGCCAGACGTTACCCTGCACGGGAACAGCGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FDC1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0PXC9

References