Detailed information of TA system TA221269

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 565834..566564
• Replicon: chromosome
• Accession: NZ_CP085774
• Organism: Enterobacter hormaechei strain 48947CZ

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A837FIL2
• Locus tag: LJU36_RS02695
• Protein ID: WP_023302825.1
• Coordinates: 565834..566148 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LJU36_RS02700
• Protein ID: WP_032620773.1
• Coordinates: 566148..566564 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJU36_RS02670 (LJU36_02670)	561500..562585	+	1086	Protein_511	cellulase family glycosylhydrolase	-
LJU36_RS02675 (LJU36_02675)	562491..563675	-	1185	WP_015570118.1	multidrug efflux MFS transporter EmrD	-
LJU36_RS02680 (LJU36_02680)	563851..564684	-	834	WP_015570117.1	DMT family transporter	-
LJU36_RS02685 (LJU36_02685)	564747..565193	-	447	WP_003861064.1	GNAT family N-acetyltransferase	-
LJU36_RS02695 (LJU36_02695)	565834..566148	+	315	WP_023302825.1	type II toxin-antitoxin system HigB family toxin	Toxin
LJU36_RS02700 (LJU36_02700)	566148..566564	+	417	WP_032620773.1	helix-turn-helix domain-containing protein	Antitoxin
LJU36_RS02705 (LJU36_02705)	566711..566809	+	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
LJU36_RS02710 (LJU36_02710)	566916..568604	+	1689	WP_023302827.1	acetolactate synthase large subunit	-
LJU36_RS02715 (LJU36_02715)	568608..568895	+	288	WP_015570113.1	acetolactate synthase small subunit	-
LJU36_RS02720 (LJU36_02720)	569021..569614	+	594	WP_003861054.1	transcriptional regulator UhpA	-
LJU36_RS02725 (LJU36_02725)	569611..571116	+	1506	WP_017694010.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12415.51 Da
• Isoelectric Point: 10.2825

>T221269 WP_023302825.1 NZ_CP085774:565834-566148 [Enterobacter hormaechei]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T221269 NZ_CP117369:13420-13725 [Salmonella enterica subsp. enterica serovar Dublin]
ATGCAGTTTAAGGTTTACACCTGTAAAAGGGAGAGTCGCTACCGGCTGTTTGTGGACGTGCAGAGCGACATCATTGACAC
CCCCGGACGGCGGATGGCTGTCCCGCTGGTCAGCGCCCGTCTGCTGTCGGAAAAGGTTCCCCGCGATCTTTACCCGGTGA
TGCATATCGGGGATGAGCCTTACCGCCTGCTGACCACGGATATGACCAGTGTGCCGGCCACCGTCATCGGGGAAGAGGTG
GCCGATCTCAGCCTCCGGGAGAACGATATCAAAAACGCCATTAACCTGATGTTCCGGGGGATCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15249.77 Da
• Isoelectric Point: 5.2068

>AT221269 WP_032620773.1 NZ_CP085774:566148-566564 [Enterobacter hormaechei]
MIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISHYEANGPEIVALRKEMESVPVGI
AVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 417 bp

>AT221269 NZ_CP117369:13200-13418 [Salmonella enterica subsp. enterica serovar Dublin]
ATGAAGCAGCGAATTACAGTGACAGTGGACAGCGACAGCTATCAGCTGCTCAAGGCATACGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGAGCCGAACGCTGGCAGGAAGAAAATCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATAGAGGCTAACGGATCGTTTGCTGACGACAACAGGAACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A837FIL2