Detailed information of TA system TA221177

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 44407..45134
• Replicon: plasmid p60214CZ_1
• Accession: NZ_CP085744
• Organism: Enterobacter hormaechei strain 60214CZ

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LJU47_RS22185
• Protein ID: WP_079899846.1
• Coordinates: 44823..45134 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LJU47_RS22180
• Protein ID: WP_227793207.1
• Coordinates: 44407..44826 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJU47_RS25105	39682..39867	+	186	WP_126336318.1	host cell division inhibitor Icd-like protein	-
LJU47_RS22150 (LJU47_22150)	39913..40089	+	177	WP_164558562.1	hypothetical protein	-
LJU47_RS22155 (LJU47_22155)	40145..40978	-	834	WP_227793204.1	hypothetical protein	-
LJU47_RS22160 (LJU47_22160)	41297..41950	+	654	WP_126336316.1	maturation control protein	-
LJU47_RS22165 (LJU47_22165)	42010..42339	+	330	WP_126336314.1	baseplate protein	-
LJU47_RS22170 (LJU47_22170)	42332..43528	+	1197	WP_227793205.1	phage tail protein	-
LJU47_RS22175 (LJU47_22175)	43503..44315	-	813	WP_227793206.1	hypothetical protein	-
LJU47_RS22180 (LJU47_22180)	44407..44826	-	420	WP_227793207.1	helix-turn-helix domain-containing protein	Antitoxin
LJU47_RS22185 (LJU47_22185)	44823..45134	-	312	WP_079899846.1	type II toxin-antitoxin system HigB family toxin	Toxin
LJU47_RS22190 (LJU47_22190)	45277..46071	+	795	WP_227793208.1	phage tail protein	-
LJU47_RS22195 (LJU47_22195)	46068..46835	+	768	WP_182403680.1	baseplate	-
LJU47_RS22200 (LJU47_22200)	46839..47819	+	981	WP_227793164.1	tail length tape measure protein	-
LJU47_RS22205 (LJU47_22205)	47816..48448	+	633	WP_227793165.1	phage baseplate assembly protein V	-
LJU47_RS22210 (LJU47_22210)	48508..49413	+	906	WP_227793166.1	recombination-associated protein RdgC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..90803	90803

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12389.15 Da
• Isoelectric Point: 9.8984

>T221177 WP_079899846.1 NZ_CP085744:c45134-44823 [Enterobacter hormaechei]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMRTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T221177 NZ_CP117354:c4236440-4236156 [Salmonella enterica subsp. enterica serovar Dublin]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGATTAA
GAAAAAGTTGGCTGATGTGCTATTGGACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGAAGTTGTGATTGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15395.50 Da
• Isoelectric Point: 4.4508

>AT221177 WP_227793207.1 NZ_CP085744:c44826-44407 [Enterobacter hormaechei]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEMPV
GVALLRTLIDQHKLSYSDLKEEIGSKSLVSQILSGQRSLTIAHIKALSSRFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT221177 NZ_CP117354:c4236672-4236430 [Salmonella enterica subsp. enterica serovar Dublin]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCACTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA