Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 124467..125194 | Replicon | plasmid p59062CZ_IncFIB |
| Accession | NZ_CP085732 | ||
| Organism | Klebsiella pneumoniae strain 59062CZ | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A7W3D9W1 |
| Locus tag | LJU45_RS28295 | Protein ID | WP_011251285.1 |
| Coordinates | 124883..125194 (-) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | LJU45_RS28290 | Protein ID | WP_011251286.1 |
| Coordinates | 124467..124886 (-) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LJU45_RS28265 (LJU45_28265) | 120059..121426 | + | 1368 | WP_227788546.1 | formimidoylglutamate deiminase | - |
| LJU45_RS28270 (LJU45_28270) | 121951..122486 | + | 536 | Protein_130 | transposase | - |
| LJU45_RS28275 (LJU45_28275) | 122600..122866 | - | 267 | WP_017900868.1 | hypothetical protein | - |
| LJU45_RS28280 (LJU45_28280) | 122970..123938 | + | 969 | WP_074168518.1 | IS5-like element IS903B family transposase | - |
| LJU45_RS28285 (LJU45_28285) | 123984..124397 | - | 414 | WP_017901337.1 | hypothetical protein | - |
| LJU45_RS28290 (LJU45_28290) | 124467..124886 | - | 420 | WP_011251286.1 | helix-turn-helix domain-containing protein | Antitoxin |
| LJU45_RS28295 (LJU45_28295) | 124883..125194 | - | 312 | WP_011251285.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| LJU45_RS28300 (LJU45_28300) | 125399..125836 | - | 438 | Protein_136 | DDE-type integrase/transposase/recombinase | - |
| LJU45_RS28305 (LJU45_28305) | 125950..126327 | + | 378 | WP_004118218.1 | transposase | - |
| LJU45_RS28310 (LJU45_28310) | 126324..126671 | + | 348 | WP_032430752.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| LJU45_RS28315 (LJU45_28315) | 126720..128258 | + | 1539 | WP_017901237.1 | IS66 family transposase | - |
| LJU45_RS28320 (LJU45_28320) | 128298..128477 | + | 180 | Protein_140 | IS5/IS1182 family transposase | - |
| LJU45_RS28325 (LJU45_28325) | 129023..130003 | - | 981 | WP_012569499.1 | IS5-like element ISKpn26 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | catA1 / tet(D) | - | 1..227661 | 227661 | |
| - | inside | IScluster/Tn | - | - | 122268..130003 | 7735 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12346.12 Da Isoelectric Point: 9.7248
>T221141 WP_011251285.1 NZ_CP085732:c125194-124883 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
Download Length: 312 bp
>T221141 NZ_CP117348:c4074918-4074631 [Salmonella enterica subsp. enterica serovar Heidelberg]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGTTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCCCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGTTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCCCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA
Antitoxin
Download Length: 140 a.a. Molecular weight: 15377.46 Da Isoelectric Point: 4.4420
>AT221141 WP_011251286.1 NZ_CP085732:c124886-124467 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
Download Length: 420 bp
>AT221141 NZ_CP117348:c4074644-4074342 [Salmonella enterica subsp. enterica serovar Heidelberg]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|