Detailed information of TA system TA221083

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4670095..4670697
• Replicon: chromosome
• Accession: NZ_CP085696
• Organism: Salmonella enterica subsp. enterica serovar Saintpaul strain S25

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C0Q3J8
• Locus tag: LJN58_RS22925
• Protein ID: WP_001159630.1
• Coordinates: 4670386..4670697 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LJN58_RS22920
• Protein ID: WP_000362050.1
• Coordinates: 4670095..4670385 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJN58_RS22905 (4667588)	4667588..4668490	+	903	WP_000331362.1	formate dehydrogenase subunit beta	-
LJN58_RS22910 (4668487)	4668487..4669122	+	636	WP_000829028.1	formate dehydrogenase cytochrome b556 subunit	-
LJN58_RS22915 (4669119)	4669119..4670048	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
LJN58_RS22920 (4670095)	4670095..4670385	-	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
LJN58_RS22925 (4670386)	4670386..4670697	-	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
LJN58_RS22930 (4670915)	4670915..4671844	+	930	WP_001127703.1	alpha/beta hydrolase	-
LJN58_RS22935 (4671930)	4671930..4672241	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
LJN58_RS22940 (4672238)	4672238..4672684	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
LJN58_RS22945 (4672699)	4672699..4673640	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
LJN58_RS22950 (4673685)	4673685..4674122	-	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
LJN58_RS22955 (4674119)	4674119..4674991	-	873	WP_000921427.1	virulence factor BrkB family protein	-
LJN58_RS22960 (4674985)	4674985..4675584	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12326.27 Da
• Isoelectric Point: 9.4460

>T221083 WP_001159630.1 NZ_CP085696:c4670697-4670386 [Salmonella enterica subsp. enterica serovar Saintpaul]
MQFIETELFTEDVKKLLDDDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T221083 NZ_CP117340:3543430-3543648 [Salmonella enterica subsp. enterica serovar Dublin]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT221083 WP_000362050.1 NZ_CP085696:c4670385-4670095 [Salmonella enterica subsp. enterica serovar Saintpaul]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT221083 NZ_CP117340:3543028-3543402 [Salmonella enterica subsp. enterica serovar Dublin]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A0R9NS64