Detailed information of TA system TA221046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4768295..4768897
• Replicon: chromosome
• Accession: NZ_CP085638
• Organism: Escherichia coli strain fEC.1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: LJX82_RS23000
• Protein ID: WP_000897305.1
• Coordinates: 4768295..4768606 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LJX82_RS23005
• Protein ID: WP_000356397.1
• Coordinates: 4768607..4768897 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJX82_RS22970 (4763325)	4763325..4764110	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
LJX82_RS22975 (4764209)	4764209..4764808	+	600	WP_001295269.1	glucose-1-phosphatase	-
LJX82_RS22980 (4764802)	4764802..4765674	+	873	WP_000920762.1	virulence factor BrkB family protein	-
LJX82_RS22985 (4765671)	4765671..4766108	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
LJX82_RS22990 (4766153)	4766153..4767094	+	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
LJX82_RS22995 (4767158)	4767158..4768066	-	909	WP_001162704.1	alpha/beta hydrolase	-
LJX82_RS23000 (4768295)	4768295..4768606	+	312	WP_000897305.1	hypothetical protein	Toxin
LJX82_RS23005 (4768607)	4768607..4768897	+	291	WP_000356397.1	NadS family protein	Antitoxin
LJX82_RS23010 (4769502)	4769502..4769720	+	219	WP_001295676.1	CopG family transcriptional regulator	-
LJX82_RS23015 (4769939)	4769939..4770181	+	243	WP_227536578.1	protein YiiF	-
LJX82_RS23020 (4770511)	4770511..4771440	-	930	WP_001749031.1	formate dehydrogenase accessory protein FdhE	-
LJX82_RS23025 (4771437)	4771437..4772072	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LJX82_RS23030 (4772069)	4772069..4772971	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T221046 WP_000897305.1 NZ_CP085638:4768295-4768606 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T221046 NZ_CP117336:c987619-987092 [Salmonella enterica subsp. enterica serovar Dublin]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTACAACGCCATGCACTACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATATGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGCGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT221046 WP_000356397.1 NZ_CP085638:4768607-4768897 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT221046 NZ_CP117336:c987906-987616 [Salmonella enterica subsp. enterica serovar Dublin]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGACTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7