Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 434688..435307 Replicon chromosome
Accession NC_009485
Organism Bradyrhizobium sp. BTAi1

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag BBTA_RS02085 Protein ID WP_038313318.1
Coordinates 435011..435307 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag BBTA_RS02080 Protein ID WP_038313315.1
Coordinates 434688..434996 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBTA_RS02050 430423..431562 + 1140 WP_012040766.1 ROK family protein -
BBTA_RS02055 432049..432708 + 660 WP_012040767.1 porin family protein -
BBTA_RS38465 433004..434626 - 1623 WP_012040768.1 recombinase family protein -
BBTA_RS02080 434688..434996 - 309 WP_038313315.1 HigA family addiction module antidote protein Antitoxin
BBTA_RS02085 435011..435307 - 297 WP_038313318.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BBTA_RS40605 435368..435613 - 246 WP_038313322.1 hypothetical protein -
BBTA_RS02095 435603..435821 - 219 WP_012040772.1 AlpA family transcriptional regulator -
BBTA_RS02100 435951..438857 - 2907 WP_012040773.1 Ti-type conjugative transfer relaxase TraA -
BBTA_RS02105 439029..439355 + 327 WP_012040774.1 conjugal transfer protein TraD -
BBTA_RS02110 439357..439575 + 219 WP_012040775.1 conjugal transfer protein TraD -
BBTA_RS02115 439697..440272 + 576 Protein_415 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 434291..482263 47972


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-97)

Antitoxin

Cro/C1-type helix-turn-helix domain

(18-69)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11480.96 Da        Isoelectric Point: 6.7268

>T22104 WP_038313318.1 NC_009485:c435307-435011 [Bradyrhizobium sp. BTAi1]
MIVGFRDEWLRAFFVDDAHSRNIPSDLEARLFRKLQMIDDATTDQDLLVPPSNHFEKLKGNLAGLRSIRVNKQYRLVFRW
DGERGEAGGIYLDDHSYR

Download         Length: 297 bp

>T22104 NC_009485:c435307-435011 [Bradyrhizobium sp. BTAi1]
ATGATCGTTGGCTTTCGGGATGAATGGTTGCGGGCTTTCTTCGTGGACGACGCCCACTCCCGCAACATCCCATCCGACCT
GGAAGCCAGGTTGTTCCGCAAGCTCCAGATGATTGACGACGCCACGACCGATCAGGACTTGCTTGTGCCACCCAGCAATC
ATTTCGAGAAGCTGAAAGGCAATCTGGCGGGTCTCCGCTCGATCCGCGTCAACAAGCAATACCGGCTGGTCTTTCGCTGG
GATGGTGAACGCGGCGAGGCCGGCGGAATCTATCTGGACGATCACAGCTACCGATGA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11548.37 Da        Isoelectric Point: 10.7465

>AT22104 WP_038313315.1 NC_009485:c434996-434688 [Bradyrhizobium sp. BTAi1]
MLTTKRKPASVGEILTEEFMEPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILARVFGNSPDFWLNVQRRNDLW
EVMNTPKERERVERARPVKKAA

Download         Length: 309 bp

>AT22104 NC_009485:c434996-434688 [Bradyrhizobium sp. BTAi1]
ATGCTGACCACCAAGCGCAAGCCGGCGAGCGTCGGCGAGATACTGACCGAAGAGTTCATGGAGCCGATGGGACTGACGCA
GGGGGCGCTCGCCGAGGCGATGGGCGTCCAGCGCAAACACGTCAACGAACTGTGCGGCAACCGCAGAAACGTGACGGCCG
CGACGGCGCTGATCCTGGCCCGTGTGTTCGGCAACAGCCCCGACTTCTGGCTCAACGTGCAGCGGCGTAACGATCTTTGG
GAGGTGATGAATACACCCAAGGAGCGCGAACGGGTCGAGCGCGCGCGTCCCGTGAAGAAGGCTGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References