Detailed information of TA system TA221027

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2056774..2057145
• Replicon: chromosome
• Accession: NZ_CP085638
• Organism: Escherichia coli strain fEC.1

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: LJX82_RS09960
• Protein ID: WP_001317028.1
• Coordinates: 2056951..2057145 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2056774..2056952 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJX82_RS09935 (2052729)	2052729..2054102	+	1374	WP_000123739.1	ATP-dependent RNA helicase DbpA	-
LJX82_RS09940 (2054231)	2054231..2055166	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
LJX82_RS09945 (2055218)	2055218..2056453	-	1236	WP_000040858.1	site-specific integrase	-
LJX82_RS09950 (2056455)	2056455..2056670	-	216	WP_000079604.1	excisionase XisR	-
- (2056774)	2056774..2056952	+	179	NuclAT_0	-	Antitoxin
- (2056774)	2056774..2056952	+	179	NuclAT_0	-	Antitoxin
- (2056774)	2056774..2056952	+	179	NuclAT_0	-	Antitoxin
- (2056774)	2056774..2056952	+	179	NuclAT_0	-	Antitoxin
LJX82_RS09955 (2056749)	2056749..2056958	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
LJX82_RS09960 (2056951)	2056951..2057145	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
LJX82_RS09965 (2057202)	2057202..2058011	-	810	WP_000166319.1	recombination protein RecT	-
LJX82_RS09970 (2058004)	2058004..2060595	-	2592	WP_000105142.1	exodeoxyribonuclease VIII	-
LJX82_RS09975 (2060697)	2060697..2060972	-	276	WP_000632297.1	protein RacC	-
LJX82_RS09980 (2061047)	2061047..2061217	-	171	WP_001352098.1	YdaE family protein	-
LJX82_RS09985 (2061217)	2061217..2061438	-	222	WP_000560225.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T221027 WP_001317028.1 NZ_CP085638:c2057145-2056951 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T221027 NZ_CP117332:317010-317378 [Salmonella enterica subsp. enterica serovar Muenster]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
TATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGCACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGGTCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGCGCCTGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT221027 NZ_CP085638:2056774-2056952 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin