Detailed information of TA system TA221023

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4555888..4556490
• Replicon: chromosome
• Accession: NZ_CP085637
• Organism: Escherichia coli strain fECg99.1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: LJU33_RS21900
• Protein ID: WP_000897305.1
• Coordinates: 4556179..4556490 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LJU33_RS21895
• Protein ID: WP_000356397.1
• Coordinates: 4555888..4556178 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJU33_RS21870 (4551813)	4551813..4552715	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
LJU33_RS21875 (4552712)	4552712..4553347	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LJU33_RS21880 (4553344)	4553344..4554273	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
LJU33_RS21885 (4554603)	4554603..4554845	-	243	WP_001087409.1	protein YiiF	-
LJU33_RS21890 (4555065)	4555065..4555283	-	219	WP_001315930.1	CopG family transcriptional regulator	-
LJU33_RS21895 (4555888)	4555888..4556178	-	291	WP_000356397.1	NadS family protein	Antitoxin
LJU33_RS21900 (4556179)	4556179..4556490	-	312	WP_000897305.1	hypothetical protein	Toxin
LJU33_RS21905 (4556719)	4556719..4557627	+	909	WP_001162704.1	alpha/beta hydrolase	-
LJU33_RS21910 (4557691)	4557691..4558632	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
LJU33_RS21915 (4558677)	4558677..4559114	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
LJU33_RS21920 (4559111)	4559111..4559983	-	873	WP_000920762.1	virulence factor BrkB family protein	-
LJU33_RS21925 (4559977)	4559977..4560576	-	600	WP_001295269.1	glucose-1-phosphatase	-
LJU33_RS21930 (4560675)	4560675..4561460	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T221023 WP_000897305.1 NZ_CP085637:c4556490-4556179 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T221023 NZ_CP117329:4557269-4557580 [Salmonella enterica subsp. enterica serovar Muenster]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATAAAGAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT221023 WP_000356397.1 NZ_CP085637:c4556178-4555888 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT221023 NZ_CP117329:4557577-4558023 [Salmonella enterica subsp. enterica serovar Muenster]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACAGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7