Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3694027..3694568 Replicon chromosome
Accession NZ_CP085637
Organism Escherichia coli strain fECg99.1

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag LJU33_RS17865 Protein ID WP_000615976.1
Coordinates 3694290..3694568 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag LJU33_RS17860 Protein ID WP_000729704.1
Coordinates 3694027..3694287 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJU33_RS17835 (3689810) 3689810..3690595 - 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -
LJU33_RS17840 (3690567) 3690567..3692279 + 1713 Protein_3487 flagellar biosynthesis protein FlhA -
LJU33_RS17845 (3692384) 3692384..3692662 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
LJU33_RS17850 (3692655) 3692655..3693011 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
LJU33_RS17855 (3693068) 3693068..3693841 - 774 WP_000543897.1 C40 family peptidase -
LJU33_RS17860 (3694027) 3694027..3694287 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LJU33_RS17865 (3694290) 3694290..3694568 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LJU33_RS17870 (3694724) 3694724..3695464 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LJU33_RS17875 (3695435) 3695435..3696202 - 768 WP_000333380.1 class II glutamine amidotransferase -
LJU33_RS17880 (3696408) 3696408..3696986 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T221018 WP_000615976.1 NZ_CP085637:3694290-3694568 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T221018 NZ_CP117329:3412996-3413214 [Salmonella enterica subsp. enterica serovar Muenster]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACAATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT221018 WP_000729704.1 NZ_CP085637:3694027-3694287 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT221018 NZ_CP117329:3412594-3412968 [Salmonella enterica subsp. enterica serovar Muenster]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCTCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References